Microba Research Discovery Report

Taxonomic Profiles (Species)

The charts below show the taxonomic composition of the analysed samples using different quantitative visualization techniques. Only the top most abundant species are shown.

Areachart

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Barchart clustered within each group

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All features barchart clustered within each group

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Scaled heatmap of most abundant features ordered by study group

Features were filtered by mean abundance. Samples were first ordered by study group and then clustered within each study group. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

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Heatmap of most abundant features ordered by study group

Features were filtered by mean abundance. Samples were first ordered by study group and then clustered within each study group. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

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Scaled heatmap of all features

Profiles were clustered by hierarchical clustering. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

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Heatmap of all features

Profiles were clustered by hierarchical clustering. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

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Scaled heatmap of top most abundant features filtered by mean abundance

Profiles were clustered by hierarchical clustering. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Heatmap of top most abundant features filtered by mean abundance

Profiles were clustered by hierarchical clustering. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Scaled heatmap of top most abundant features filtered by max abundance

Profiles were clustered by hierarchical clustering. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Heatmap of top most abundant features filtered by max abundance

Profiles were clustered by hierarchical clustering. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Scaled heatmap of top most variable features

Profiles were clustered by hierarchical clustering. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

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Hierarchically clustered barchart

Profiles were clustered by hierarchical clustering.

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Interactive Barchart

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Microbial alpha diversity (species)

This page provides an overview of the microbial alpha diversity of the analysed samples. Alpha diversity is measured by the Shannon index and species richnes. Richness simply quantifies the total number of species present in each sample. Shannon index additionally accounts for relative abundance and evenness of the species present and quantifies the entropy of microbial communties. Barcharts and boxplots present the mean diversity in each study group.

Methods:

Shannon diversity was compared using linear mixed effect regression, including Participant as a random effect (intercept), and Treatment as a fixed effect. Effect tested: Treatment. Richness was compared using linear mixed effect regression, including Participant as a random effect (intercept), and Treatment as a fixed effect. Effect tested: Treatment. Data was rarefied to 702268 reads.

Index: Richness

Index: Shannon index

Summary Table

Index	rarefiedTo	P lmer condition effect	Mean Pos	Mean Abundance	Median Abundance	Mean Treatmentpost_treatment	Median Treatmentpost_treatment	SD Treatmentpost_treatment	Mean Treatmentpre_treatment	Median Treatmentpre_treatment	SD Treatmentpre_treatment	Fold Change Log2(Treatmentpre_treatment/Treatmentpost_treatment)	Positive samples	Positive Treatmentpost_treatment	Positive Treatmentpre_treatment	Positive_Treatmentpost_treatment_percent	Positive_Treatmentpre_treatment_percent
Shannon	702268	0.093	3	3	3	3	3	0.35	3.1	3	0.5	0.047	38 / 38 (100%)	19 / 19 (100%)	19 / 19 (100%)	1	1
Richness	702268	0.021	45	45	36	43	36	18	48	37	25	0.16	38 / 38 (100%)	19 / 19 (100%)	19 / 19 (100%)	1	1

Download diversity values in csv format . On some platforms (including Windows) you may need to change the suffix from .txt or .html to .csv before opening the file in a spreadsheet program, like Excel.

Clustering and ordination (species)

Taxonomic profiles were analyzed using supervised and unsupervised multivariate methods. Profiles were ordinated using the unsupervised methods Principal Coordinates Analysis (PCoA), Non-Metric Multidimensional Scaling (NMDS) and Principal Component Analysis (PCA). PCoA and NMDS are related to PCA, but take dissimlarity matrices as input. PCoA and NMDS both attempt to represent the pairwise dissimlarities between samples in low dimensional space as close as possible. NMDS is a rank-based approach and therefore less effected by outliers.

The supervised methods Adonis and Redundancy analysis (RDA) were used to assess if variance in microbial community composition can be attributed to the study condition. NMDS, PCoA and Adonis were run on Bray-Curtis dissimilarities. A short introduction of the used methods can be found at the GUide to STatistical Analysis in Microbial Ecology (GUSTA ME). Sparse Partial Least Square Discriminant Analysis (sPLS-DA) from the MixMc package was additionaly used to extract features associated with the study condition.

Unsupervised ordination

Interactive PCA (clr transformed)

Please click here to view an interactive 3D PCA.

Univariate analysis of species abundance

Differentially abundant species were identified by ANOVA or LMER (linear mixed effect regression) of clr transformed relative abudances, Fisher's exact test and/or ALDEx2 (on species read counts). Fisher's exact test is used to test for differences in the detection rate, i.e. number of samples in which each species has been detected.

LMER is used for repeated measures data, using random effects to control for correlation between samples from the same subject. Fixed effects are included for treatment groups, time, and treatment over time, where appropriate. The LMER P values correspond to a nested model test of the significance of including the corresponding fixed effect.

ALDEx2 uses subsampling (Bayesian sampling) to estimate the underlying technical variation. For each subsample instance, center log-ratio transformed data is statistically compared across study groups and computed P values are corrected for multiple testing using the Benjamini–Hochberg procedure. The expected P value (mean P value) is reported, which are those that would likely have been observed if the same samples had been run multiple times. The expected values are reported for both the distribution of P values and for the distribution of Benjamini–Hochberg corrected values.

Taxon	GTDB taxonomy	P lmer condition effect (sqrt)	FDR lmer condition effect (sqrt)	Pbonf lmer condition effect (sqrt)	P lmer condition effect (clr)	FDR lmer condition effect (clr)	Pbonf lmer condition effect (clr)	Mean Pos	Mean Abundance	Median Abundance	Mean Treatmentpost_treatment	Median Treatmentpost_treatment	SD Treatmentpost_treatment	Mean Treatmentpre_treatment	Median Treatmentpre_treatment	SD Treatmentpre_treatment	Fold Change Log2(Treatmentpre_treatment/Treatmentpost_treatment)	Positive samples	Positive Treatmentpost_treatment	Positive Treatmentpre_treatment	Positive_Treatmentpost_treatment_percent	Positive_Treatmentpre_treatment_percent
s__Acetatifactor sp900066365	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Acetatifactor	0.7	0.84	1	0.76	0.86	1	0.3	0.032	0	0.042	0	0.13	0.021	0	0.07	-1	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Acetatifactor sp900066565	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Acetatifactor	0.3	0.64	1	0.57	0.74	1	0.69	0.11	0	0.15	0	0.42	0.065	0	0.2	-1.2	6 / 38 (16%)	3 / 19 (16%)	3 / 19 (16%)	0.158	0.158
s__Adlercreutzia equolifaciens	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Eggerthellaceae; g__Adlercreutzia	0.82	0.94	1	0.8	0.88	1	0.22	0.023	0	0.029	0	0.099	0.017	0	0.054	-0.77	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Agathobacter MIC6414	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Agathobacter	0.3	0.64	1	0.012	0.45	1	0.84	0.067	0	0.13	0	0.52	0.0053	0	0.023	-4.6	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Agathobacter faecis	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Agathobacter	0.37	0.66	1	0.51	0.71	1	2.7	1.4	0.49	1.1	0	1.8	1.7	0.51	2.5	0.63	20 / 38 (53%)	9 / 19 (47%)	11 / 19 (58%)	0.474	0.579
s__Agathobacter rectale	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Agathobacter	0.35	0.66	1	0.56	0.73	1	9.7	8.1	6.5	8.7	6.3	7.1	7.6	6.7	6.8	-0.2	32 / 38 (84%)	16 / 19 (84%)	16 / 19 (84%)	0.842	0.842
s__Agathobaculum butyriciproducens	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Butyricicoccaceae; g__Agathobaculum	0.035	0.52	1	0.019	0.45	1	0.61	0.16	0	0.25	0	0.38	0.072	0	0.22	-1.8	10 / 38 (26%)	8 / 19 (42%)	2 / 19 (11%)	0.421	0.105
s__Akkermansia muciniphila	d__Bacteria; p__Verrucomicrobiota; c__Verrucomicrobiae; o__Verrucomicrobiales; f__Akkermansiaceae; g__Akkermansia	0.54	0.77	1	0.92	0.95	1	1.8	0.28	0	0.2	0	0.59	0.37	0	0.95	0.89	6 / 38 (16%)	3 / 19 (16%)	3 / 19 (16%)	0.158	0.158
s__Alistipes finegoldii	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Rikenellaceae; g__Alistipes	0.077	0.59	1	0.17	0.6	1	0.61	0.064	0	0.034	0	0.15	0.094	0	0.3	1.5	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Alistipes onderdonkii	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Rikenellaceae; g__Alistipes	0.45	0.7	1	0.29	0.62	1	0.94	0.074	0	0.11	0	0.4	0.04	0	0.17	-1.5	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Alistipes putredinis	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Rikenellaceae; g__Alistipes	0.55	0.77	1	0.15	0.6	1	1.3	0.3	0	0.35	0	1.2	0.25	0	0.53	-0.49	9 / 38 (24%)	3 / 19 (16%)	6 / 19 (32%)	0.158	0.316
s__Anaerostipes hadrus	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Anaerostipes	0.016	0.52	1	0.27	0.62	1	3.4	2.9	1.8	3.7	2.8	3.7	2	1.6	1.8	-0.89	32 / 38 (84%)	16 / 19 (84%)	16 / 19 (84%)	0.842	0.842
s__Anaerostipes hadrus_A	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Anaerostipes	0.72	0.86	1	0.74	0.85	1	2.3	0.6	0	0.75	0	1.6	0.46	0	1.1	-0.71	10 / 38 (26%)	5 / 19 (26%)	5 / 19 (26%)	0.263	0.263
s__Bacteroides caccae	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Bacteroides	0.26	0.62	1	0.13	0.6	1	0.88	0.12	0	0.092	0	0.4	0.14	0	0.36	0.61	5 / 38 (13%)	1 / 19 (5.3%)	4 / 19 (21%)	0.0526	0.211
s__Bacteroides ovatus	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Bacteroides	0.19	0.62	1	0.3	0.62	1	0.99	0.13	0	0.22	0	0.78	0.036	0	0.11	-2.6	5 / 38 (13%)	3 / 19 (16%)	2 / 19 (11%)	0.158	0.105
s__Bacteroides stercoris	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Bacteroides	0.99	0.99	1	0.91	0.95	1	1.7	0.36	0	0.36	0	0.8	0.36	0	0.89	0	8 / 38 (21%)	4 / 19 (21%)	4 / 19 (21%)	0.211	0.211
s__Bacteroides uniformis	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Bacteroides	0.2	0.62	1	0.21	0.6	1	4.5	1.4	0	2.1	0	6.5	0.74	0	1.5	-1.5	12 / 38 (32%)	7 / 19 (37%)	5 / 19 (26%)	0.368	0.263
s__Bacteroides_B massiliensis	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Bacteroides_B	0.34	0.66	1	0.21	0.6	1	0.44	0.059	0	0.044	0	0.19	0.073	0	0.18	0.73	5 / 38 (13%)	1 / 19 (5.3%)	4 / 19 (21%)	0.0526	0.211
s__Bacteroides_B vulgatus	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Bacteroides_B	0.32	0.66	1	0.17	0.6	1	2.7	0.84	0	0.69	0	2.2	0.99	0	1.9	0.52	12 / 38 (32%)	4 / 19 (21%)	8 / 19 (42%)	0.211	0.421
s__Barnesiella intestinihominis	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Barnesiellaceae; g__Barnesiella	0.25	0.62	1	0.4	0.68	1	0.31	0.025	0	0.0066	0	0.029	0.043	0	0.14	2.7	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Bifidobacterium MIC6680	d__Bacteria; p__Actinobacteriota; c__Actinobacteria; o__Actinomycetales; f__Bifidobacteriaceae; g__Bifidobacterium	0.61	0.8	1	0.28	0.62	1	1.2	0.095	0	0.11	0	0.37	0.082	0	0.36	-0.42	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Bifidobacterium MIC7686	d__Bacteria; p__Actinobacteriota; c__Actinobacteria; o__Actinomycetales; f__Bifidobacteriaceae; g__Bifidobacterium	0.62	0.81	1	0.28	0.62	1	0.64	0.05	0	0.056	0	0.19	0.045	0	0.19	-0.32	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Bifidobacterium adolescentis	d__Bacteria; p__Actinobacteriota; c__Actinobacteria; o__Actinomycetales; f__Bifidobacteriaceae; g__Bifidobacterium	0.36	0.66	1	0.081	0.6	1	6.8	3.8	0.74	3.7	1.1	5.6	3.8	0	11	0.038	21 / 38 (55%)	12 / 19 (63%)	9 / 19 (47%)	0.632	0.474
s__Bifidobacterium angulatum	d__Bacteria; p__Actinobacteriota; c__Actinobacteria; o__Actinomycetales; f__Bifidobacteriaceae; g__Bifidobacterium	0.92	0.97	1	0.63	0.79	1	1.1	0.15	0	0.16	0	0.48	0.13	0	0.4	-0.3	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__Bifidobacterium bifidum	d__Bacteria; p__Actinobacteriota; c__Actinobacteria; o__Actinomycetales; f__Bifidobacteriaceae; g__Bifidobacterium	0.59	0.78	1	0.48	0.71	1	0.43	0.056	0	0.063	0	0.15	0.049	0	0.16	-0.36	5 / 38 (13%)	3 / 19 (16%)	2 / 19 (11%)	0.158	0.105
s__Bifidobacterium longum	d__Bacteria; p__Actinobacteriota; c__Actinobacteria; o__Actinomycetales; f__Bifidobacteriaceae; g__Bifidobacterium	0.05	0.52	1	0.027	0.45	1	3.5	2.7	1.7	2.5	2.4	2.1	2.9	1.4	5.1	0.21	29 / 38 (76%)	14 / 19 (74%)	15 / 19 (79%)	0.737	0.789
s__Bifidobacterium pseudocatenulatum	d__Bacteria; p__Actinobacteriota; c__Actinobacteria; o__Actinomycetales; f__Bifidobacteriaceae; g__Bifidobacterium	0.43	0.69	1	0.47	0.71	1	3.6	1.3	0	0.7	0	1.3	1.9	0	4.2	1.4	14 / 38 (37%)	7 / 19 (37%)	7 / 19 (37%)	0.368	0.368
s__Blautia sp000436935	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia	0.25	0.62	1	0.19	0.6	1	1.8	0.24	0	0.35	0	1.2	0.12	0	0.35	-1.5	5 / 38 (13%)	3 / 19 (16%)	2 / 19 (11%)	0.158	0.105
s__Blautia_A MIC7077	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.45	0.7	1	0.66	0.81	1	0.28	0.029	0	0.029	0	0.086	0.03	0	0.09	0.049	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Blautia_A MIC8050	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.42	0.69	1	0.16	0.6	1	0.91	0.14	0	0.15	0	0.36	0.13	0	0.42	-0.21	6 / 38 (16%)	4 / 19 (21%)	2 / 19 (11%)	0.211	0.105
s__Blautia_A MIC8343	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.18	0.62	1	0.12	0.6	1	0.18	0.023	0	0.019	0	0.084	0.027	0	0.057	0.51	5 / 38 (13%)	1 / 19 (5.3%)	4 / 19 (21%)	0.0526	0.211
s__Blautia_A MIC9663	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.33	0.66	1	0.2	0.6	1	0.42	0.033	0	0.046	0	0.14	0.02	0	0.089	-1.2	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Blautia_A massiliensis	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.13	0.62	1	0.29	0.62	1	2.1	1.3	0.5	1	0.5	1.3	1.7	0.5	2.5	0.77	24 / 38 (63%)	12 / 19 (63%)	12 / 19 (63%)	0.632	0.632
s__Blautia_A obeum	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.14	0.62	1	0.32	0.64	1	1.8	1	0.4	1.3	0.53	1.6	0.74	0.36	1.1	-0.81	21 / 38 (55%)	11 / 19 (58%)	10 / 19 (53%)	0.579	0.526
s__Blautia_A sp000285855	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.44	0.7	1	0.48	0.71	1	0.26	0.047	0	0.06	0	0.13	0.034	0	0.093	-0.82	7 / 38 (18%)	4 / 19 (21%)	3 / 19 (16%)	0.211	0.158
s__Blautia_A sp000436615	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.39	0.66	1	0.13	0.6	1	1.1	0.17	0	0.14	0	0.36	0.19	0	0.51	0.44	6 / 38 (16%)	3 / 19 (16%)	3 / 19 (16%)	0.158	0.158
s__Blautia_A sp900066145	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.96	0.98	1	0.2	0.6	1	0.84	0.066	0	0.049	0	0.18	0.083	0	0.36	0.76	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Blautia_A sp900066165	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.91	0.97	1	0.77	0.86	1	1.5	0.96	0.69	1	0.58	1.2	0.88	0.78	0.8	-0.18	25 / 38 (66%)	12 / 19 (63%)	13 / 19 (68%)	0.632	0.684
s__Blautia_A sp900066205	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.3	0.64	1	0.12	0.6	1	1.3	0.17	0	0.16	0	0.71	0.17	0	0.43	0.087	5 / 38 (13%)	1 / 19 (5.3%)	4 / 19 (21%)	0.0526	0.211
s__Blautia_A sp900066335	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.67	0.83	1	0.62	0.79	1	0.87	0.16	0	0.22	0	0.71	0.11	0	0.26	-1	7 / 38 (18%)	3 / 19 (16%)	4 / 19 (21%)	0.158	0.211
s__Blautia_A sp900066355	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.61	0.8	1	0.68	0.83	1	0.23	0.03	0	0.023	0	0.073	0.036	0	0.092	0.65	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__Blautia_A sp900120195	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.46	0.71	1	0.24	0.62	1	0.21	0.017	0	0.021	0	0.069	0.012	0	0.054	-0.81	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Blautia_A wexlerae	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Blautia_A	0.55	0.77	1	0.73	0.85	1	9.5	9.5	7.7	9.5	5.9	8.4	9.6	8.4	6.6	0.015	38 / 38 (100%)	19 / 19 (100%)	19 / 19 (100%)	1	1
s__CAG-103 MIC7540	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__CAG-103	0.34	0.66	1	0.59	0.75	1	0.56	0.074	0	0.049	0	0.16	0.098	0	0.26	1	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__CAG-103 sp000432375	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__CAG-103	0.25	0.62	1	0.0059	0.45	1	0.66	0.12	0	0.15	0	0.34	0.098	0	0.24	-0.61	7 / 38 (18%)	4 / 19 (21%)	3 / 19 (16%)	0.211	0.158
s__CAG-110 sp000435995	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__CAG-110	0.26	0.62	1	0.21	0.6	1	0.18	0.014	0	0.025	0	0.085	0.0037	0	0.016	-2.8	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__CAG-110 sp003525905	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__CAG-110	0.31	0.65	1	0.22	0.6	1	0.37	0.029	0	0.036	0	0.11	0.022	0	0.097	-0.71	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__CAG-127 sp900319515	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__CAG-127	0.96	0.98	1	0.76	0.85	1	0.54	0.071	0	0.089	0	0.34	0.054	0	0.13	-0.72	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__CAG-138 MIC9630	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Christensenellales; f__CAG-138; g__CAG-138 MIC9630	0.038	0.52	1	0.063	0.54	1	1.3	0.17	0	0.045	0	0.2	0.3	0	0.65	2.7	5 / 38 (13%)	1 / 19 (5.3%)	4 / 19 (21%)	0.0526	0.211
s__CAG-1427 sp000435675	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Eggerthellaceae; g__CAG-1427	0.89	0.97	1	0.82	0.89	1	0.21	0.022	0	0.025	0	0.08	0.019	0	0.058	-0.4	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__CAG-1427 sp000436075	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Eggerthellaceae; g__CAG-1427	0.17	0.62	1	0.19	0.6	1	0.46	0.049	0	0.025	0	0.11	0.072	0	0.17	1.5	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__CAG-170 sp000432135	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__CAG-170	0.19	0.62	1	0.24	0.61	1	1.5	0.12	0	0.0063	0	0.027	0.23	0	0.9	5.2	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-177 sp003514385	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__CAG-177	0.57	0.78	1	0.22	0.61	1	1.3	0.17	0	0.16	0	0.39	0.17	0	0.55	0.087	5 / 38 (13%)	3 / 19 (16%)	2 / 19 (11%)	0.158	0.105
s__CAG-217 sp000436335	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__CAG-217	0.89	0.97	1	0.3	0.62	1	1.6	0.42	0	0.38	0	0.71	0.45	0	0.94	0.24	10 / 38 (26%)	6 / 19 (32%)	4 / 19 (21%)	0.316	0.211
s__CAG-245 sp000435175	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__TANB77; f__CAG-508; g__CAG-245	0.27	0.62	1	0.33	0.64	1	0.48	0.05	0	0.02	0	0.085	0.081	0	0.24	2	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__CAG-274 MIC6942	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__CAG-274; g__CAG-274	0.15	0.62	1	0.11	0.6	1	0.33	0.026	0	0.048	0	0.17	0.0046	0	0.02	-3.4	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__CAG-274 sp000432155	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__CAG-274; g__CAG-274	0.21	0.62	1	0.17	0.6	1	0.6	0.079	0	0.12	0	0.32	0.043	0	0.14	-1.5	5 / 38 (13%)	3 / 19 (16%)	2 / 19 (11%)	0.158	0.105
s__CAG-302 sp001916775	d__Bacteria; p__Firmicutes; c__Bacilli; o__RF39; f__CAG-302; g__CAG-302	0.28	0.62	1	0.45	0.7	1	0.83	0.11	0	0.077	0	0.23	0.14	0	0.43	0.86	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__CAG-313 sp000433035	d__Bacteria; p__Firmicutes; c__Bacilli; o__ML615J-28; f__CAG-313; g__CAG-313	0.17	0.62	1	0.42	0.68	1	0.46	0.037	0	0.023	0	0.1	0.05	0	0.18	1.1	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-354 sp001915925	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__TANB77; f__CAG-508; g__CAG-354	0.92	0.97	1	0.83	0.89	1	0.41	0.054	0	0.057	0	0.17	0.051	0	0.14	-0.16	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__CAG-41 sp900066215	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Monoglobales; f__UBA1381; g__CAG-41	0.033	0.52	1	0.041	0.45	1	0.79	0.37	0	0.47	0.37	0.7	0.28	0	0.5	-0.75	18 / 38 (47%)	11 / 19 (58%)	7 / 19 (37%)	0.579	0.368
s__CAG-417 sp000432835	d__Bacteria; p__Firmicutes; c__Bacilli; o__RF39; f__CAG-611; g__CAG-417	0.68	0.84	1	0.3	0.62	1	1.2	0.092	0	0.091	0	0.29	0.094	0	0.41	0.047	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__CAG-452 sp000434035	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__TANB77; f__CAG-508; g__CAG-452	0.24	0.62	1	0.41	0.68	1	0.29	0.023	0	0.012	0	0.051	0.034	0	0.1	1.5	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-460 MIC6729	d__Bacteria; p__Firmicutes; c__Bacilli; o__RF39; f__CAG-1000; g__CAG-460	0.21	0.62	1	0.36	0.66	1	2	0.16	0	0.069	0	0.3	0.25	0	0.76	1.9	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-485 MIC8260	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Muribaculaceae; g__CAG-485	0.7	0.84	1	0.57	0.74	1	0.49	0.039	0	0.043	0	0.19	0.035	0	0.1	-0.3	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-533 sp000434495	d__Bacteria; p__Firmicutes; c__Bacilli; o__RF39; f__CAG-1000; g__CAG-533	0.19	0.62	1	0.34	0.66	1	2	0.15	0	0.055	0	0.24	0.25	0	0.76	2.2	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-557 sp000435275	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__CAG-557	0.46	0.71	1	0.49	0.71	1	0.63	0.049	0	0.043	0	0.19	0.056	0	0.17	0.38	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-56 sp900066615	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__CAG-56	0.51	0.75	1	0.26	0.62	1	0.96	0.53	0.26	0.54	0.39	0.62	0.53	0	0.88	-0.027	21 / 38 (55%)	12 / 19 (63%)	9 / 19 (47%)	0.632	0.474
s__CAG-628 sp003524085	d__Bacteria; p__Firmicutes; c__Bacilli; o__RF39; f__CAG-1000; g__CAG-628	0.22	0.62	1	0.39	0.68	1	0.47	0.037	0	0.018	0	0.08	0.056	0	0.17	1.6	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-83 sp000431575	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__CAG-83	0.37	0.66	1	0.25	0.62	1	0.53	0.042	0	0.06	0	0.2	0.023	0	0.1	-1.4	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__CAG-83 sp000435555	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__CAG-83	0.22	0.62	1	0.37	0.66	1	1	0.083	0	0.035	0	0.15	0.13	0	0.39	1.9	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-83 sp003487665	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__CAG-83	0.24	0.62	1	0.4	0.68	1	0.45	0.035	0	0.018	0	0.078	0.053	0	0.16	1.6	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__CAG-877 sp000433455	d__Bacteria; p__Firmicutes; c__Bacilli; o__RF39; f__CAG-611; g__CAG-877	0.32	0.66	1	0.25	0.62	1	0.41	0.043	0	0.03	0	0.095	0.056	0	0.2	0.9	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Clostridium MIC8163	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Clostridiales; f__Clostridiaceae; g__Clostridium	0.65	0.82	1	0.69	0.84	1	0.71	0.13	0	0.059	0	0.14	0.2	0	0.71	1.8	7 / 38 (18%)	4 / 19 (21%)	3 / 19 (16%)	0.211	0.158
s__Collinsella MIC6390	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Collinsella	0.33	0.66	1	0.065	0.54	1	1.5	0.12	0	0.11	0	0.38	0.12	0	0.53	0.13	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Collinsella MIC7086	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Collinsella	0.39	0.66	1	0.46	0.71	1	0.7	0.055	0	0.043	0	0.19	0.068	0	0.2	0.66	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Collinsella MIC7241	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Collinsella	0.19	0.62	1	0.2	0.6	1	1.3	0.14	0	0.038	0	0.17	0.24	0	0.78	2.7	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Collinsella MIC7504	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Collinsella	0.12	0.62	1	0.2	0.6	1	0.75	0.079	0	0.049	0	0.21	0.11	0	0.31	1.2	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Collinsella aerofaciens_F	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Collinsella	0.2	0.62	1	0.2	0.6	1	0.81	0.064	0	0.11	0	0.33	0.019	0	0.084	-2.5	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Collinsella sp002232035	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Collinsella	0.41	0.67	1	0.66	0.81	1	3.9	0.52	0	0.25	0	0.79	0.79	0	2.1	1.7	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__Collinsella sp003487125	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Collinsella	0.91	0.97	1	0.78	0.87	1	0.58	0.077	0	0.085	0	0.28	0.069	0	0.19	-0.3	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__Coprococcus eutactus_A	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Coprococcus	0.13	0.62	1	0.031	0.45	1	1.9	0.45	0	0.57	0	1.2	0.32	0	0.79	-0.83	9 / 38 (24%)	5 / 19 (26%)	4 / 19 (21%)	0.263	0.211
s__Coprococcus sp900066115	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Coprococcus	0.55	0.77	1	0.24	0.61	1	2.5	0.33	0	0.35	0	0.85	0.32	0	1.3	-0.13	5 / 38 (13%)	3 / 19 (16%)	2 / 19 (11%)	0.158	0.105
s__Coprococcus_A MIC9199	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Coprococcus_A	0.31	0.65	1	0.017	0.45	1	0.33	0.026	0	0.033	0	0.099	0.019	0	0.082	-0.8	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Coprococcus_A catus	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Coprococcus_A	0.68	0.84	1	0.48	0.71	1	0.42	0.078	0	0.081	0	0.18	0.074	0	0.2	-0.13	7 / 38 (18%)	4 / 19 (21%)	3 / 19 (16%)	0.211	0.158
s__Coprococcus_B comes	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Coprococcus_B	0.097	0.62	1	0.24	0.62	1	0.76	0.56	0.59	0.46	0.52	0.42	0.65	0.63	0.5	0.5	28 / 38 (74%)	13 / 19 (68%)	15 / 19 (79%)	0.684	0.789
s__Dialister invisus	d__Bacteria; p__Firmicutes_C; c__Negativicutes; o__Veillonellales; f__Dialisteraceae; g__Dialister	0.95	0.98	1	0.41	0.68	1	2	0.37	0	0.34	0	0.82	0.4	0	1	0.23	7 / 38 (18%)	4 / 19 (21%)	3 / 19 (16%)	0.211	0.158
s__Dialister sp900343095	d__Bacteria; p__Firmicutes_C; c__Negativicutes; o__Veillonellales; f__Dialisteraceae; g__Dialister	0.55	0.77	1	0.86	0.91	1	1	0.19	0	0.15	0	0.41	0.24	0	0.49	0.68	7 / 38 (18%)	3 / 19 (16%)	4 / 19 (21%)	0.158	0.211
s__Dorea formicigenerans	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Dorea	0.25	0.62	1	0.16	0.6	1	0.65	0.29	0	0.34	0.23	0.4	0.24	0	0.37	-0.5	17 / 38 (45%)	10 / 19 (53%)	7 / 19 (37%)	0.526	0.368
s__Dorea longicatena	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Dorea	0.23	0.62	1	0.97	0.97	1	1.2	0.52	0	0.43	0	0.59	0.62	0	0.95	0.53	17 / 38 (45%)	9 / 19 (47%)	8 / 19 (42%)	0.474	0.421
s__Dorea longicatena_B	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Dorea	0.99	0.99	1	0.41	0.68	1	1	0.49	0	0.45	0.29	0.66	0.52	0	0.75	0.21	18 / 38 (47%)	10 / 19 (53%)	8 / 19 (42%)	0.526	0.421
s__Dorea sp000433215	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Dorea	0.078	0.59	1	0.14	0.6	1	0.2	0.021	0	0.0049	0	0.022	0.038	0	0.092	3	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Dorea sp900066555	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Dorea	0.28	0.63	1	0.27	0.62	1	0.18	0.019	0	0.012	0	0.051	0.025	0	0.068	1.1	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Duncaniella MIC6738	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Muribaculaceae; g__Duncaniella	0.66	0.83	1	0.56	0.73	1	0.49	0.039	0	0.041	0	0.18	0.036	0	0.11	-0.19	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__ER4 sp900317525	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__ER4	0.94	0.98	1	0.36	0.66	1	0.51	0.067	0	0.057	0	0.15	0.077	0	0.24	0.43	5 / 38 (13%)	3 / 19 (16%)	2 / 19 (11%)	0.158	0.105
s__Eisenbergiella sp900066775	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Eisenbergiella	0.17	0.62	1	0.35	0.66	1	2	0.15	0	0.048	0	0.21	0.26	0	1.1	2.4	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Erysipelatoclostridium ramosum	d__Bacteria; p__Firmicutes; c__Bacilli; o__Erysipelotrichales; f__Erysipelatoclostridiaceae; g__Erysipelatoclostridium	0.15	0.62	1	0.37	0.66	1	0.37	0.029	0	0.012	0	0.051	0.047	0	0.17	2	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Erysipelatoclostridium sp000752095	d__Bacteria; p__Firmicutes; c__Bacilli; o__Erysipelotrichales; f__Erysipelatoclostridiaceae; g__Erysipelatoclostridium	0.038	0.52	1	0.017	0.45	1	0.92	0.7	0.46	0.56	0.43	0.7	0.84	0.47	1.1	0.58	29 / 38 (76%)	12 / 19 (63%)	17 / 19 (89%)	0.632	0.895
s__Eubacterium_E hallii	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Eubacterium_E	0.44	0.69	1	0.35	0.66	1	2	1.8	1.7	1.8	1.7	1.2	1.7	1.4	1.1	-0.082	33 / 38 (87%)	16 / 19 (84%)	17 / 19 (89%)	0.842	0.895
s__Eubacterium_E hallii_A	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Eubacterium_E	0.062	0.52	1	0.049	0.47	1	0.65	0.15	0	0.22	0	0.4	0.089	0	0.23	-1.3	9 / 38 (24%)	6 / 19 (32%)	3 / 19 (16%)	0.316	0.158
s__Eubacterium_F MIC7104	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Eubacterium_F	0.051	0.52	1	0.069	0.55	1	0.44	0.058	0	0.015	0	0.067	0.1	0	0.21	2.7	5 / 38 (13%)	1 / 19 (5.3%)	4 / 19 (21%)	0.0526	0.211
s__Eubacterium_G ventriosum	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Eubacterium_G	0.014	0.52	1	0.018	0.45	1	0.65	0.12	0	0.052	0	0.23	0.19	0	0.33	1.9	7 / 38 (18%)	1 / 19 (5.3%)	6 / 19 (32%)	0.0526	0.316
s__Eubacterium_I ramulus	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Eubacterium_I	0.094	0.62	1	0.16	0.6	1	0.58	0.12	0	0.078	0	0.2	0.17	0	0.36	1.1	8 / 38 (21%)	3 / 19 (16%)	5 / 19 (26%)	0.158	0.263
s__Eubacterium_I ramulus_A	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Eubacterium_I	0.38	0.66	1	0.49	0.71	1	0.82	0.11	0	0.07	0	0.23	0.15	0	0.42	1.1	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__Eubacterium_R sp000434995	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__Eubacterium_R	0.26	0.62	1	0.33	0.64	1	1.5	0.23	0	0.17	0	0.55	0.3	0	0.61	0.82	6 / 38 (16%)	2 / 19 (11%)	4 / 19 (21%)	0.105	0.211
s__F23-B02 sp002472405	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__F23-B02	0.13	0.62	1	0.16	0.6	1	0.73	0.077	0	0.025	0	0.11	0.13	0	0.32	2.4	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Faecalibacterium MIC7145	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.1	0.62	1	0.092	0.6	1	0.6	0.14	0	0.076	0	0.21	0.21	0	0.38	1.5	9 / 38 (24%)	3 / 19 (16%)	6 / 19 (32%)	0.158	0.316
s__Faecalibacterium MIC7301	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.47	0.71	1	0.54	0.73	1	0.23	0.018	0	0.016	0	0.071	0.021	0	0.062	0.39	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Faecalibacterium MIC8666	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.18	0.62	1	0.5	0.71	1	1.3	0.14	0	0.083	0	0.3	0.19	0	0.75	1.2	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Faecalibacterium MIC9210	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.18	0.62	1	0.14	0.6	1	3	0.4	0	0.48	0	1.9	0.31	0	1.2	-0.63	5 / 38 (13%)	3 / 19 (16%)	2 / 19 (11%)	0.158	0.105
s__Faecalibacterium prausnitzii_A	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.62	0.81	1	0.75	0.85	1	1.2	0.093	0	0.15	0	0.65	0.037	0	0.12	-2	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Faecalibacterium prausnitzii_C	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.95	0.98	1	0.5	0.71	1	3.9	2.5	0.8	2.7	0.75	3.9	2.3	0.83	3.3	-0.23	24 / 38 (63%)	11 / 19 (58%)	13 / 19 (68%)	0.579	0.684
s__Faecalibacterium prausnitzii_D	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.87	0.97	1	0.28	0.62	1	2.3	1.1	0	1	0.43	1.5	1.1	0	1.7	0.14	18 / 38 (47%)	10 / 19 (53%)	8 / 19 (42%)	0.526	0.421
s__Faecalibacterium prausnitzii_G	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.2	0.62	1	0.04	0.45	1	1.6	0.75	0	0.83	0.51	0.99	0.67	0	1.1	-0.31	18 / 38 (47%)	11 / 19 (58%)	7 / 19 (37%)	0.579	0.368
s__Faecalibacterium prausnitzii_I	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.99	0.99	1	0.95	0.96	1	0.45	0.095	0	0.1	0	0.23	0.089	0	0.21	-0.17	8 / 38 (21%)	4 / 19 (21%)	4 / 19 (21%)	0.211	0.211
s__Faecalibacterium prausnitzii_J	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.29	0.64	1	0.22	0.6	1	0.8	0.17	0	0.13	0	0.36	0.2	0	0.5	0.62	8 / 38 (21%)	3 / 19 (16%)	5 / 19 (26%)	0.158	0.263
s__Faecalibacterium prausnitzii_K	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Faecalibacterium	0.59	0.78	1	0.73	0.85	1	1.8	0.6	0	0.79	0	2	0.41	0	0.74	-0.95	13 / 38 (34%)	7 / 19 (37%)	6 / 19 (32%)	0.368	0.316
s__Faecalicatena faecis	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Faecalicatena	0.0082	0.52	1	0.2	0.6	1	1.4	0.62	0	0.4	0	0.67	0.83	0	1.2	1.1	17 / 38 (45%)	8 / 19 (42%)	9 / 19 (47%)	0.421	0.474
s__Faecalicatena gnavus	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Faecalicatena	0.08	0.59	1	0.16	0.6	1	1.3	0.13	0	0.043	0	0.19	0.22	0	0.79	2.4	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Faecalicatena lactaris	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Faecalicatena	0.06	0.52	1	0.18	0.6	1	0.93	0.2	0	0.13	0	0.36	0.26	0	0.7	1	8 / 38 (21%)	3 / 19 (16%)	5 / 19 (26%)	0.158	0.263
s__Faecalicatena torques	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Faecalicatena	0.017	0.52	1	0.026	0.45	1	0.42	0.099	0	0.027	0	0.085	0.17	0	0.26	2.7	9 / 38 (24%)	2 / 19 (11%)	7 / 19 (37%)	0.105	0.368
s__Fusicatenibacter saccharivorans	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Fusicatenibacter	0.37	0.66	1	0.12	0.6	1	4.5	4	2.7	4.2	3	3.8	3.8	2.6	4.1	-0.14	34 / 38 (89%)	17 / 19 (89%)	17 / 19 (89%)	0.895	0.895
s__GCA-900066135 MIC6659	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__GCA-900066135	0.056	0.52	1	0.027	0.45	1	0.32	0.068	0	0.09	0	0.15	0.047	0	0.18	-0.94	8 / 38 (21%)	6 / 19 (32%)	2 / 19 (11%)	0.316	0.105
s__Gemmiger MIC9530	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Gemmiger	0.69	0.84	1	0.72	0.85	1	0.37	0.059	0	0.064	0	0.17	0.054	0	0.13	-0.25	6 / 38 (16%)	3 / 19 (16%)	3 / 19 (16%)	0.158	0.158
s__Gemmiger formicilis	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Gemmiger	0.039	0.52	1	0.034	0.45	1	2.2	0.77	0	1.1	0	2.4	0.42	0	1.1	-1.4	13 / 38 (34%)	9 / 19 (47%)	4 / 19 (21%)	0.474	0.211
s__Gemmiger sp003476825	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Gemmiger	0.8	0.94	1	0.66	0.81	1	1.8	1.3	0.94	1.3	0.89	1.4	1.3	0.98	1.7	0	29 / 38 (76%)	15 / 19 (79%)	14 / 19 (74%)	0.789	0.737
s__Holdemanella sp002299315	d__Bacteria; p__Firmicutes; c__Bacilli; o__Erysipelotrichales; f__Erysipelotrichaceae; g__Holdemanella	0.48	0.71	1	0.28	0.62	1	2.5	0.26	0	0.21	0	0.62	0.31	0	1	0.56	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Intestinibacter bartlettii	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Peptostreptococcales; f__Peptostreptococcaceae; g__Intestinibacter	0.87	0.97	1	0.85	0.9	1	0.27	0.05	0	0.049	0	0.12	0.05	0	0.12	0.029	7 / 38 (18%)	3 / 19 (16%)	4 / 19 (21%)	0.158	0.211
s__KLE1615 sp900066985	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__KLE1615	0.42	0.69	1	0.45	0.7	1	1.2	0.55	0	0.52	0	0.76	0.58	0	0.74	0.16	17 / 38 (45%)	8 / 19 (42%)	9 / 19 (47%)	0.421	0.474
s__Lachnospira eligens_B	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Lachnospira	0.2	0.62	1	0.29	0.62	1	0.37	0.039	0	0.011	0	0.047	0.067	0	0.17	2.6	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Lachnospira rogosae	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Lachnospira	0.85	0.95	1	0.84	0.9	1	0.57	0.075	0	0.11	0	0.43	0.04	0	0.1	-1.5	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__Lachnospira sp000437735	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Lachnospira	0.2	0.62	1	0.15	0.6	1	0.49	0.039	0	0.065	0	0.2	0.013	0	0.057	-2.3	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Lachnospira sp003451515	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Lachnospira	0.93	0.98	1	0.45	0.7	1	1.2	0.21	0	0.18	0	0.48	0.24	0	0.62	0.42	7 / 38 (18%)	4 / 19 (21%)	3 / 19 (16%)	0.211	0.158
s__Lactobacillus_B salivarius	d__Bacteria; p__Firmicutes; c__Bacilli; o__Lactobacillales; f__Lactobacillaceae; g__Lactobacillus_B	0.15	0.62	1	0.13	0.6	1	0.81	0.064	0	0.11	0	0.34	0.022	0	0.098	-2.3	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Megamonas funiformis	d__Bacteria; p__Firmicutes_C; c__Negativicutes; o__Selenomonadales; f__Selenomonadaceae; g__Megamonas	0.83	0.94	1	0.97	0.97	1	0.46	0.049	0	0.063	0	0.19	0.035	0	0.13	-0.85	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Methanobrevibacter_A smithii	d__Archaea; p__Euryarchaeota; c__Methanobacteria; o__Methanobacteriales; f__Methanobacteriaceae; g__Methanobrevibacter_A	0.18	0.62	1	0.36	0.66	1	1	0.083	0	0.025	0	0.11	0.14	0	0.42	2.5	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Monoglobus pectinilyticus	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Monoglobales; f__Monoglobaceae; g__Monoglobus	0.062	0.52	1	0.09	0.6	1	0.2	0.038	0	0.0091	0	0.027	0.066	0	0.13	2.9	7 / 38 (18%)	2 / 19 (11%)	5 / 19 (26%)	0.105	0.263
s__NK3B98 sp003150485	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__NK3B98	0.4	0.67	1	0.52	0.72	1	0.2	0.016	0	0.012	0	0.054	0.019	0	0.057	0.66	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Oxalobacter formigenes_A	d__Bacteria; p__Proteobacteria; c__Gammaproteobacteria; o__Burkholderiales; f__Burkholderiaceae; g__Oxalobacter	0.23	0.62	1	0.18	0.6	1	0.11	0.0083	0	0.011	0	0.036	0.0051	0	0.022	-1.1	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Parabacteroides distasonis	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Tannerellaceae; g__Parabacteroides	0.18	0.62	1	0.23	0.61	1	0.6	0.063	0	0.057	0	0.25	0.07	0	0.24	0.3	4 / 38 (11%)	1 / 19 (5.3%)	3 / 19 (16%)	0.0526	0.158
s__Parabacteroides merdae	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Tannerellaceae; g__Parabacteroides	0.39	0.66	1	0.48	0.71	1	0.27	0.05	0	0.04	0	0.12	0.061	0	0.14	0.61	7 / 38 (18%)	3 / 19 (16%)	4 / 19 (21%)	0.158	0.211
s__Parasutterella excrementihominis	d__Bacteria; p__Proteobacteria; c__Gammaproteobacteria; o__Burkholderiales; f__Burkholderiaceae; g__Parasutterella	0.26	0.62	1	0.17	0.6	1	0.46	0.036	0	0.059	0	0.19	0.013	0	0.055	-2.2	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Phascolarctobacterium faecium	d__Bacteria; p__Firmicutes_C; c__Negativicutes; o__Acidaminococcales; f__Acidaminococcaceae; g__Phascolarctobacterium	0.031	0.52	1	0.26	0.62	1	0.82	0.19	0	0.15	0	0.49	0.24	0	0.62	0.68	9 / 38 (24%)	4 / 19 (21%)	5 / 19 (26%)	0.211	0.263
s__Prevotella copri	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Prevotella	0.48	0.71	1	0.92	0.95	1	5.9	0.62	0	1.1	0	3.3	0.14	0	0.45	-3	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Prevotella sp000436915	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Prevotella	0.59	0.78	1	0.54	0.73	1	0.45	0.035	0	0.036	0	0.16	0.035	0	0.1	-0.041	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Prevotella sp900313215	d__Bacteria; p__Bacteroidota; c__Bacteroidia; o__Bacteroidales; f__Bacteroidaceae; g__Prevotella	0.84	0.94	1	0.75	0.85	1	3.3	0.35	0	0.5	0	1.6	0.19	0	0.57	-1.4	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__QAMH01 MIC6543	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__QAMH01; g__QAMH01	0.27	0.62	1	0.42	0.68	1	0.5	0.039	0	0.022	0	0.094	0.057	0	0.17	1.4	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Romboutsia timonensis	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Peptostreptococcales; f__Peptostreptococcaceae; g__Romboutsia	0.83	0.94	1	0.93	0.95	1	0.73	0.23	0	0.2	0	0.35	0.26	0	0.51	0.38	12 / 38 (32%)	6 / 19 (32%)	6 / 19 (32%)	0.316	0.316
s__Roseburia hominis	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Roseburia	0.05	0.52	1	0.083	0.6	1	0.84	0.11	0	0.042	0	0.18	0.18	0	0.43	2.1	5 / 38 (13%)	1 / 19 (5.3%)	4 / 19 (21%)	0.0526	0.211
s__Roseburia intestinalis	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Roseburia	0.041	0.52	1	0.049	0.47	1	0.57	0.12	0	0.048	0	0.14	0.19	0	0.34	2	8 / 38 (21%)	2 / 19 (11%)	6 / 19 (32%)	0.105	0.316
s__Ruminiclostridium_C MIC9168	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__Ruminiclostridium_C	0.18	0.62	1	0.18	0.6	1	0.3	0.023	0	0.032	0	0.11	0.014	0	0.062	-1.2	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Ruminiclostridium_C sp000435295	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__Ruminiclostridium_C	0.024	0.52	1	0.039	0.45	1	0.22	0.057	0	0.028	0	0.075	0.086	0	0.13	1.6	10 / 38 (26%)	3 / 19 (16%)	7 / 19 (37%)	0.158	0.368
s__Ruminiclostridium_E MIC9645	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Ruminiclostridium_E	0.19	0.62	1	0.34	0.66	1	1.5	0.12	0	0.041	0	0.18	0.2	0	0.59	2.3	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Ruminococcus_A sp003011855	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__Ruminococcus_A	0.8	0.94	1	0.75	0.85	1	0.93	0.37	0	0.36	0	0.64	0.38	0	0.83	0.078	15 / 38 (39%)	8 / 19 (42%)	7 / 19 (37%)	0.421	0.368
s__Ruminococcus_C callidus	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Ruminococcus_C	0.38	0.66	1	0.42	0.68	1	2	0.16	0	0.11	0	0.34	0.2	0	0.87	0.86	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Ruminococcus_C sp000433635	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Ruminococcus_C	0.75	0.89	1	0.75	0.85	1	0.77	0.14	0	0.13	0	0.34	0.15	0	0.4	0.21	7 / 38 (18%)	3 / 19 (16%)	4 / 19 (21%)	0.158	0.211
s__Ruminococcus_C sp000980705	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Ruminococcus_C	0.43	0.69	1	0.45	0.7	1	0.98	0.077	0	0.12	0	0.36	0.036	0	0.16	-1.7	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Ruminococcus_D bicirculans	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__Ruminococcus_D	0.64	0.82	1	0.95	0.96	1	2.6	1.3	0.11	1.5	0	2.6	1	0.22	1.5	-0.58	19 / 38 (50%)	9 / 19 (47%)	10 / 19 (53%)	0.474	0.526
s__Ruminococcus_E bromii	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__Ruminococcus_E	0.88	0.97	1	0.05	0.47	1	3.7	0.39	0	0.4	0	1.4	0.39	0	1.2	-0.037	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__Ruminococcus_E bromii_B	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__Ruminococcus_E	0.1	0.62	1	0.094	0.6	1	4.6	2.6	0.57	2.2	0	3.5	2.9	0.73	3.8	0.4	21 / 38 (55%)	9 / 19 (47%)	12 / 19 (63%)	0.474	0.632
s__Ruminococcus_E sp900314705	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__Ruminococcus_E	0.75	0.89	1	0.71	0.85	1	1.6	0.13	0	0.2	0	0.85	0.056	0	0.17	-1.8	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Senegalimassilia anaerobia	d__Bacteria; p__Actinobacteriota; c__Coriobacteriia; o__Coriobacteriales; f__Eggerthellaceae; g__Senegalimassilia	0.38	0.66	1	0.53	0.73	1	0.31	0.025	0	0.019	0	0.083	0.031	0	0.092	0.71	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__Streptococcus salivarius	d__Bacteria; p__Firmicutes; c__Bacilli; o__Lactobacillales; f__Streptococcaceae; g__Streptococcus	0.014	0.52	1	0.016	0.45	1	0.65	0.2	0	0.3	0	0.5	0.11	0	0.29	-1.4	12 / 38 (32%)	8 / 19 (42%)	4 / 19 (21%)	0.421	0.211
s__Streptococcus thermophilus	d__Bacteria; p__Firmicutes; c__Bacilli; o__Lactobacillales; f__Streptococcaceae; g__Streptococcus	0.54	0.77	1	0.88	0.92	1	1.1	0.24	0	0.14	0	0.46	0.33	0	1	1.2	8 / 38 (21%)	4 / 19 (21%)	4 / 19 (21%)	0.211	0.211
s__TF01-11 sp003149875	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__TF01-11	0.37	0.66	1	0.53	0.73	1	0.17	0.013	0	0.0048	0	0.021	0.022	0	0.065	2.2	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__TF01-11 sp003524945	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__TF01-11	0.27	0.62	1	0.16	0.6	1	0.19	0.015	0	0.025	0	0.086	0.0047	0	0.021	-2.4	3 / 38 (7.9%)	2 / 19 (11%)	1 / 19 (5.3%)	0.105	0.0526
s__Turicibacter sp001543345	d__Bacteria; p__Firmicutes; c__Bacilli; o__Haloplasmatales; f__Turicibacteraceae; g__Turicibacter	0.38	0.66	1	0.52	0.72	1	0.22	0.017	0	0.0079	0	0.034	0.027	0	0.096	1.8	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__UBA11524 sp000437595	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Christensenellales; f__CAG-74; g__UBA11524	0.23	0.62	1	0.43	0.7	1	1.5	0.12	0	0.082	0	0.36	0.15	0	0.5	0.87	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__UBA11774 sp003507655	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__UBA11774	0.81	0.94	1	0.31	0.62	1	1.3	0.37	0	0.3	0	0.6	0.44	0	1.1	0.55	11 / 38 (29%)	6 / 19 (32%)	5 / 19 (26%)	0.316	0.263
s__UBA1191 MIC6696	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Peptostreptococcales; f__Anaerovoracaceae; g__UBA1191	0.58	0.78	1	0.55	0.73	1	0.58	0.046	0	0.049	0	0.21	0.042	0	0.15	-0.22	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__UBA1394 sp900066845	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Ruminococcaceae; g__UBA1394	0.65	0.82	1	0.81	0.89	1	0.46	0.06	0	0.082	0	0.25	0.039	0	0.1	-1.1	5 / 38 (13%)	2 / 19 (11%)	3 / 19 (16%)	0.105	0.158
s__UBA1417 sp003531055	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__UBA1417	0.021	0.52	1	0.032	0.45	1	0.62	0.099	0	0.024	0	0.1	0.17	0	0.35	2.8	6 / 38 (16%)	1 / 19 (5.3%)	5 / 19 (26%)	0.0526	0.263
s__UBA1691 MIC9213	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Acutalibacteraceae; g__UBA1691	0.21	0.62	1	0.58	0.74	1	1	0.11	0	0.086	0	0.31	0.13	0	0.4	0.6	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105
s__UBA1777 MIC6732	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Oscillospirales; f__Oscillospiraceae; g__UBA1777	0.24	0.62	1	0.39	0.68	1	1.2	0.093	0	0.047	0	0.2	0.14	0	0.41	1.6	3 / 38 (7.9%)	1 / 19 (5.3%)	2 / 19 (11%)	0.0526	0.105
s__UBA7160 MIC6745	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__UBA7160	0.88	0.97	1	0.72	0.85	1	0.51	0.12	0	0.11	0	0.29	0.13	0	0.34	0.24	9 / 38 (24%)	5 / 19 (26%)	4 / 19 (21%)	0.263	0.211
s__UBA7160 MIC9207	d__Bacteria; p__Firmicutes_A; c__Clostridia; o__Lachnospirales; f__Lachnospiraceae; g__UBA7160	0.53	0.77	1	0.82	0.89	1	0.12	0.013	0	0.012	0	0.037	0.013	0	0.041	0.12	4 / 38 (11%)	2 / 19 (11%)	2 / 19 (11%)	0.105	0.105

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Methods:

P lmer condition effect (sqrt): Differentially abundant species were identified by linear mixed effect regression without confounders. Random effects: Participant (intercept). Fixed effects: Treatment. Effect tested: Treatment.

P lmer condition effect (clr): Differentially abundant species were identified by linear mixed effect regression without confounders. Random effects: Participant (intercept). Fixed effects: Treatment. Effect tested: Treatment.

Glossary:

clr: Centered log-ratio transformation. P Welch's ANOVA (sqrt): Welch's ANOVA p-values (Anova was run on sqrt transformed abundances). It is an alternative to the classic ANOVA and can be used even if the samples have unequal variances and/or unequal sample sizes. P Welch's t-test (sqrt): Welch's T-test p-values (run on sqrt transformed abundances). It is an alternative to the classic Student's t-test and is more reliable if the data violates the assumption of homogeneity of variances and/or if the study conditions have unequal sample sizes. P Welch's ANOVA (clr): Welch's ANOVA p-values (Anova was run on clr transformed abundances). It is an alternative to the classic ANOVA and can be used even if the samples have unequal variances and/or unequal sample sizes. P Welch's t-test (clr): Welch's T-test p-values (run on clr transformed abundances). It is an alternative to the classic Student's t-test and is more reliable if the data violates the assumption of homogeneity of variances and/or if the study conditions have unequal sample sizes. P lmer XXX (clr): P-value of Linear Mixed-Effects Regression on clr transformed abundances, testing significance of XXX effect. Varying intercepts per subjects are used to control for repeated measures. Pbonf: Bonferroni corrected p-value. FDR: False Discovery Rate q-value. Mean Pos: Mean abundance in positive samples. Positive samples: The number and percentage of samples in which each species has been detected. Positive XXX: The number and percentage of positive samples in study group XXX. Positive_XXX_percent: Percentage of positive samples in study group XXX. P Welch's t-test (Aldex2): Expected P value of Welch’s t-test computed by Aldex2. FDR Welch's t-test (Aldex2): Expected Benjamini-Hochberg corrected P value for Welch’s t-test. P Wilcoxon rank test (Aldex2): Expected P value of Wilcoxon rank test computed by Aldex2. FDR Wilcoxon rank test (Aldex2): Expected Benjamini-Hochberg corrected P value of Wilcoxon test. P Kruskal-Wallace test (Aldex2): Expected P value of Kruskal-Wallace test. FDR Kruskal-Wallace test (Aldex2): Expected Benjamini-Hochberg corrected P value of Kruskal-Wallace test. P GLM test (Aldex2): Expected P value of generalized linear model. FDR GLM test (Aldex2): Expected Benjamini-Hochberg corrected P value of generalized linear model.

Differentially abundant taxa (species)

The following plots present the distribution of the top most differentially abundant species across all applied statistical analysis. Plots are ordered alphabetically.

Glossary:

"Rel. Abundance": Relative abundance data; "Rel. Abundance (sqrt)": sqrt (Hellinger) transformed relative abundances; "Rel. Abundance (clr)": centered log-ratio (clr) transformed read counts.