Microba Research Discovery Report

Functional profiles: Membrane Transport Proteins (TCDB)

Quantitative visualisation of the top most abundant microbial functions identified in the analysed samples.

Scaled heatmap of most abundant features ordered by study group

Features were filtered by mean abundance. Samples were first ordered by study group and then clustered within each study group. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Heatmap of most abundant features ordered by study group

Features were filtered by mean abundance. Samples were first ordered by study group and then clustered within each study group. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Scaled heatmap of all features

Profiles were clustered by hierarchical clustering. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Heatmap of all features

Profiles were clustered by hierarchical clustering. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Scaled heatmap of top most abundant features filtered by mean abundance

Profiles were clustered by hierarchical clustering. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Heatmap of top most abundant features filtered by mean abundance

Profiles were clustered by hierarchical clustering. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Scaled heatmap of top most abundant features filtered by max abundance

Profiles were clustered by hierarchical clustering. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Heatmap of top most abundant features filtered by max abundance

Profiles were clustered by hierarchical clustering. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Scaled heatmap of top most variable features

Profiles were clustered by hierarchical clustering. Abundances were scaled to a max value of 1. Color scale shows not-detected (white), and abundance ranging from low (blue) to high (yellow).

Click here to open full-sized image in new window.

Membrane Transport Proteins (TCDB)

Functional Alpha Diversity

This page provides an overview of the functional alpha diversity of the analysed sample. Alpha diversity was measured using the Shannon index and functional richnes. Richness measures the total number of gene functions present in each sample. Shannon index combines richness and evenness.

Methods:

Shannon diversity was compared using a standard t-test. Richness was compared using a standard t-test. Data was rarefied to 74 reads.

Index: Richness

Index: Shannon index

Summary Table

Index	rarefiedTo	P Welch's t-test	Mean Pos	Mean Abundance	Median Abundance	Mean Post_Treatment_Fibre_Intakehigh	Median Post_Treatment_Fibre_Intakehigh	SD Post_Treatment_Fibre_Intakehigh	Mean Post_Treatment_Fibre_Intakelow	Median Post_Treatment_Fibre_Intakelow	SD Post_Treatment_Fibre_Intakelow	Fold Change Log2(Post_Treatment_Fibre_Intakelow/Post_Treatment_Fibre_Intakehigh)	Positive samples	Positive Post_Treatment_Fibre_Intakehigh	Positive Post_Treatment_Fibre_Intakelow	Positive_Post_Treatment_Fibre_Intakehigh_percent	Positive_Post_Treatment_Fibre_Intakelow_percent
Shannon	74	0.8	3.1	3.1	2.9	3	3	0.39	3.1	2.8	0.57	0.047	18 / 18 (100%)	11 / 11 (100%)	7 / 7 (100%)	1	1
Richness	74	0.61	31	31	25	30	26	12	34	24	16	0.18	18 / 18 (100%)	11 / 11 (100%)	7 / 7 (100%)	1	1

Download diversity values in csv format . On some platforms (including Windows) you may need to change the suffix from .txt or .html to .csv before opening the file in a spreadsheet program, like Excel.

Functional clustering and ordination

Microbial functions were analyzed using supervised and unsupervised multivariate techniques. Functional profiles were ordinated using the unsupervised methods Principal Coordinates Analysis (PCoA), Non-Metric Multidimensional Scaling (NMDS) and Principal Component Analysis (PCA). The supervised methods Adonis and Redundancy analysis (RDA) were used to assess if variance in the functional profiles was significantly associated with the study condition. A guide explaining these methods can be found here. Sparse Partial Least Square Discriminant Analysis (sPLS-DA) from the MixMc package was additionaly used to extract features associated with the condition of interest.

Unsupervised ordination

Interactive PCA (clr transformed)

Please click here to view an interactive 3D PCA.

Supervised Analysis

Membrane Transport Proteins (TCDB)

Univariate analysis of microbial functions

Differentially abundant microbial functions were identified using the univariate methods ANOVA or LMER (linear mixed effect regression) on clr transformed relative abundance data, Fisher's exact test, and Aldex2 (ANOVA-like Differential Expression). Aldex2 was run on read count data. Fisher's exact test was used to test for diffrerences in the presence and absence (detection rate) of microbial functions across study groups.

LMER is used for repeated measures data, using random effects to control for correlation between samples from the same subject. Fixed effects are included for treatment groups, time, and treatment over time, where appropriate. The LMER P values correspond to a nested model test of the significance of including the corresponding fixed effect.

ALDEx2 uses subsampling (Bayesian sampling) to estimate the underlying technical variation. For each subsample instance, center log-ratio transformed data is statistically compared across study groups and computed P values are corrected for multiple testing using the Benjamini–Hochberg procedure. The expected P value (mean P value) is reported, which are those that would likely have been observed if the same samples had been run multiple times. The expected values are reported for both the distribution of P values and for the distribution of Benjamini–Hochberg corrected values.

Function	Annotation	P Welch's t-test (sqrt)	FDR Welch's t-test (sqrt)	Pbonf Welch's t-test (sqrt)	Cohen's d Welch's t-test (sqrt)	P Welch's t-test (clr)	FDR Welch's t-test (clr)	Pbonf Welch's t-test (clr)	Cohen's d Welch's t-test (clr)	P Fisher's exact test	FDR Fisher's exact test	Pbonf Fisher's exact test	P Welch's t-test (ALDEx2)	FDR Welch's t-test (ALDEx2)	P Wilcoxon rank test (ALDEx2)	FDR Wilcoxon rank test (ALDEx2)	Mean Pos	Mean Abundance	Median Abundance	Mean Post_Treatment_Fibre_Intakehigh	Median Post_Treatment_Fibre_Intakehigh	SD Post_Treatment_Fibre_Intakehigh	Mean Post_Treatment_Fibre_Intakelow	Median Post_Treatment_Fibre_Intakelow	SD Post_Treatment_Fibre_Intakelow	Fold Change Log2(Post_Treatment_Fibre_Intakelow/Post_Treatment_Fibre_Intakehigh)	Positive samples	Positive Post_Treatment_Fibre_Intakehigh	Positive Post_Treatment_Fibre_Intakelow	Positive_Post_Treatment_Fibre_Intakehigh_percent	Positive_Post_Treatment_Fibre_Intakelow_percent
3.A.1.140.6	The ATP-binding Cassette (ABC) Superfamily	0.71	0.83	1	0.17	0.54	1	1	0.33	1	1	1	0.6	0.94	0.6	0.96	0.00018	3e-05	0	3.8e-05	0	1e-04	1.7e-05	0	4.5e-05	-1.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.2.1	The ATP-binding Cassette (ABC) Superfamily	0.58	0.8	1	-0.34	0.83	1	1	0.1	1	1	1	0.55	0.94	0.55	0.96	0.00046	7.7e-05	0	2.1e-05	0	4.7e-05	0.00016	0	0.00044	2.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.23.1.22	The Cyanobacterial Porin (CBP) Family	0.7	0.83	1	-0.22	0.7	1	1	0.17	1	1	1	0.55	0.94	0.57	0.96	0.00059	9.8e-05	0	4.9e-05	0	0.00013	0.00017	0	0.00046	1.8	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.A.23.2.1	The Small Conductance Mechanosensitive Ion Channel (MscS) Family	0.74	0.85	1	-0.19	0.5	1	1	0.28	1	1	1	0.53	0.94	0.55	0.96	0.00028	4.6e-05	0	2.6e-05	0	6.9e-05	7.7e-05	0	2e-04	1.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.B.1.1.2	The Na⁺-transporting Carboxylic Acid Decarboxylase (NaT-DC) Family	0.88	0.94	1	0.067	0.49	1	1	0.29	1	1	1	0.53	0.94	0.55	0.96	0.00018	4.1e-05	0	5.2e-05	0	0.00015	2.3e-05	0	5.1e-05	-1.2	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
3.A.1.106.3	The ATP-binding Cassette (ABC) Superfamily	0.44	0.8	1	0.41	0.2	1	1	0.69	0.25	1	1	0.33	0.92	0.3	0.94	0.0014	0.0011	0.00071	0.0012	0.00098	0.0011	0.00088	0.00036	0.001	-0.45	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
3.A.1.135.1	The ATP-binding Cassette (ABC) Superfamily	0.79	0.88	1	0.12	0.57	1	1	0.31	1	1	1	0.59	0.94	0.61	0.97	0.00018	3e-05	0	3.4e-05	0	7.8e-05	2.3e-05	0	6.1e-05	-0.56	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.A.35.4.1	The CorA Metal Ion Transporter (MIT) Family	0.61	0.8	1	-0.31	0.73	1	1	0.15	1	1	1	0.48	0.93	0.53	0.96	0.00023	3.8e-05	0	1.3e-05	0	3.5e-05	7.7e-05	0	2e-04	2.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.55.3.1	The Metal Ion (Mn²⁺-iron) Transporter (Nramp) Family	0.63	0.8	1	-0.29	0.65	1	1	0.2	1	1	1	0.49	0.94	0.54	0.96	0.00026	4.3e-05	0	1.6e-05	0	3.7e-05	8.7e-05	0	0.00023	2.4	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
9.B.154.1.1	The Putative Holin-2 (PH-2) Family	0.19	0.8	1	0.55	0.17	1	1	0.66	0.6	1	1	0.41	0.92	0.44	0.95	0.0014	0.00039	0	0.00061	0	0.0014	4e-05	0	0.00011	-3.9	5 / 18 (28%)	4 / 11 (36%)	1 / 7 (14%)	0.364	0.143
2.A.76.1.3	The Resistance to Homoserine/Threonine (RhtB) Family	0.8	0.88	1	-0.14	0.45	1	1	0.31	1	1	1	0.5	0.93	0.54	0.95	0.00022	3.7e-05	0	2.3e-05	0	5.2e-05	5.8e-05	0	0.00015	1.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.2.3.1	The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family	0.61	0.8	1	-0.31	0.73	1	1	0.14	1	1	1	0.55	0.94	0.56	0.96	0.00017	2.8e-05	0	9.5e-06	0	2.3e-05	5.8e-05	0	0.00015	2.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
4.A.1.1.2	The PTS Glucose-Glucoside (Glc) Family	0.18	0.8	1	-0.88	0.14	1	1	-0.94	0.25	1	1	0.4	0.91	0.43	0.93	0.00027	6.1e-05	0	1.1e-05	0	3.6e-05	0.00014	0	0.00027	3.7	4 / 18 (22%)	1 / 11 (9.1%)	3 / 7 (43%)	0.0909	0.429
3.A.1.4.1	The ATP-binding Cassette (ABC) Superfamily	0.41	0.8	1	-0.52	0.54	1	1	-0.33	1	1	1	0.51	0.94	0.53	0.95	0.00093	0.00021	0	3.4e-05	0	7.8e-05	0.00048	0	0.0012	3.8	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
8.A.46.1.1	The Glycan-binding Protein (SusD) Family	0.53	0.8	1	-0.32	0.82	1	1	0.13	0.53	1	1	0.57	0.94	0.59	0.96	4.1e-05	6.9e-06	0	6e-06	0	2e-05	8.3e-06	0	1.4e-05	0.47	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
1.B.14.1.9	The Outer Membrane Receptor (OMR) Family	0.53	0.8	1	-0.39	0.99	1	1	-0.0065	1	1	1	0.49	0.93	0.54	0.96	0.00049	8.1e-05	0	1.6e-05	0	3.7e-05	0.00018	0	0.00049	3.5	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.1.21.22	The Major Facilitator Superfamily (MFS)	0.91	0.96	1	-0.051	0.9	1	1	0.065	1	1	1	0.61	0.95	0.66	0.97	0.00021	1e-04	1.3e-05	0.00011	0	0.00027	8.9e-05	2.7e-05	0.00013	-0.31	9 / 18 (50%)	5 / 11 (45%)	4 / 7 (57%)	0.455	0.571
2.A.17.1.5	The Proton-dependent Oligopeptide Transporter (POT/PTR) Family	0.27	0.8	1	-0.71	0.27	1	1	-0.7	0.53	1	1	0.43	0.93	0.46	0.95	0.00024	4e-05	0	5.4e-06	0	1.8e-05	9.4e-05	0	2e-04	4.1	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.6.4.1	The Resistance-Nodulation-Cell Division (RND) Superfamily	0.42	0.8	1	-0.49	0.51	1	1	-0.34	1	1	1	0.5	0.93	0.55	0.95	0.00053	0.00012	0	3.4e-05	0	1e-04	0.00025	0	0.00061	2.9	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
2.A.21.3.19	The Solute:Sodium Symporter (SSS) Family	0.57	0.8	1	-0.28	0.57	1	1	-0.27	0.37	1	1	0.55	0.94	0.63	0.96	0.00087	0.00048	9.5e-05	0.00044	0	0.00077	0.00055	0.00012	0.00079	0.32	10 / 18 (56%)	5 / 11 (45%)	5 / 7 (71%)	0.455	0.714
2.A.1.1.42	The Major Facilitator Superfamily (MFS)	0.41	0.8	1	0.43	0.19	1	1	0.72	0.25	1	1	0.31	0.92	0.31	0.94	0.0013	0.001	0.00081	0.0011	0.00091	0.00096	0.00085	0.00072	0.00087	-0.37	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
3.A.1.11.7	The ATP-binding Cassette (ABC) Superfamily	0.87	0.93	1	-0.085	0.8	1	1	0.14	1	1	1	0.61	0.95	0.64	0.97	2e-04	6.6e-05	0	5.6e-05	0	1e-04	8.2e-05	0	0.00015	0.55	6 / 18 (33%)	4 / 11 (36%)	2 / 7 (29%)	0.364	0.286
8.A.1.1.3	The Membrane Fusion Protein (MFP) Family	0.29	0.8	1	-0.66	0.35	1	1	-0.55	0.53	1	1	0.52	0.94	0.56	0.96	2e-04	3.4e-05	0	8.1e-06	0	2.7e-05	7.5e-05	0	0.00015	3.2	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
8.A.42.1.6	The small hydrophilic YodL domain/protein (YodL) Family	0.93	0.96	1	-0.038	0.88	1	1	-0.079	0.37	1	1	0.71	0.96	0.74	0.98	0.00064	0.00036	0.00019	4e-04	0	0.00057	0.00028	0.00025	0.00029	-0.51	10 / 18 (56%)	5 / 11 (45%)	5 / 7 (71%)	0.455	0.714
9.B.307.1.2	The FtsH AAA(+) Zinc Metaloprotease (FtsH) Family	0.54	0.8	1	-0.39	0.99	1	1	0.0063	1	1	1	0.49	0.92	0.54	0.94	0.00046	7.7e-05	0	1.6e-05	0	3.7e-05	0.00017	0	0.00046	3.4	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.2.27	The ATP-binding Cassette (ABC) Superfamily	0.58	0.8	1	-0.34	0.89	1	1	0.066	1	1	1	0.51	0.94	0.55	0.96	0.00055	9.1e-05	0	2.6e-05	0	6.9e-05	0.00019	0	0.00051	2.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.33.1.5	The NhaA Na⁺:H⁺ Antiporter (NhaA) Family	0.97	0.98	1	0.024	0.37	1	1	0.47	0.33	1	1	0.56	0.94	0.48	0.96	0.00071	0.00051	0.00044	0.00044	0.00044	0.00034	0.00063	0.00024	0.00076	0.52	13 / 18 (72%)	9 / 11 (82%)	4 / 7 (57%)	0.818	0.571
2.A.3.1.4	The Amino Acid-Polyamine-Organocation (APC) Family	0.29	0.8	1	-0.67	0.32	1	1	-0.55	0.33	1	1	0.48	0.93	0.53	0.96	0.00023	6.3e-05	0	1.5e-05	0	3.3e-05	0.00014	0	3e-04	3.2	5 / 18 (28%)	2 / 11 (18%)	3 / 7 (43%)	0.182	0.429
3.A.1.5.1	The ATP-binding Cassette (ABC) Superfamily	0.56	0.8	1	-0.36	0.91	1	1	0.052	1	1	1	0.5	0.94	0.53	0.95	4e-04	6.6e-05	0	1.6e-05	0	3.7e-05	0.00015	0	0.00038	3.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.40.1.1	The Nucleobase/Ascorbate Transporter (NAT) or Nucleobase:Cation Symporter-2 (NCS2) Family	0.61	0.8	1	-0.3	0.71	1	1	0.17	1	1	1	0.48	0.93	0.5	0.95	0.00028	4.7e-05	0	1.6e-05	0	3.7e-05	9.7e-05	0	0.00026	2.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.48.2.5	The Curli Fiber Subunit Porin. CgsA. CsgG (CsgG) Family	0.1	0.8	1	0.7	0.088	1	1	0.86	0.6	1	1	0.4	0.93	0.4	0.94	0.00024	6.8e-05	0	0.00011	0	0.00021	4.4e-06	0	1.2e-05	-4.6	5 / 18 (28%)	4 / 11 (36%)	1 / 7 (14%)	0.364	0.143
9.A.47.2.2	The Tight Adherence (Pilus) Biogenesis Apparatus (TABA) Family	0.48	0.8	1	0.38	0.22	1	1	0.67	0.25	1	1	0.31	0.91	0.31	0.94	0.0024	0.0018	0.0015	0.002	0.0013	0.0015	0.0016	0.0017	0.0017	-0.32	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
3.A.10.1.10	The H⁺. Na⁺-translocating Pyrophosphatase (M⁺-PPase) Family	0.46	0.8	1	0.31	0.66	1	1	0.22	1	1	1	0.67	0.95	0.74	0.97	0.0011	0.00061	6.8e-05	0.00085	2.9e-05	0.0017	0.00023	0.00011	0.00035	-1.9	10 / 18 (56%)	6 / 11 (55%)	4 / 7 (57%)	0.545	0.571
2.A.13.1.1	The C₄-Dicarboxylate Uptake (Dcu) Family	0.57	0.8	1	-0.35	0.86	1	1	0.076	1	1	1	0.5	0.94	0.52	0.96	0.00022	3.6e-05	0	9.5e-06	0	2.3e-05	7.7e-05	0	2e-04	3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.37.1.1	The Monovalent Cation:Proton Antiporter-2 (CPA2) Family	0.46	0.8	1	-0.47	0.68	1	1	-0.2	1	1	1	0.51	0.94	0.54	0.96	3e-04	6.7e-05	0	1.5e-05	0	3.3e-05	0.00015	0	0.00038	3.3	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
3.A.1.7.1	The ATP-binding Cassette (ABC) Superfamily	0.37	0.8	1	-0.59	0.4	1	1	-0.48	1	1	1	0.49	0.93	0.51	0.95	0.00063	0.00014	0	1.5e-05	0	3.3e-05	0.00033	0	0.00084	4.5	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
1.A.43.3.3	The Camphor Resistance or Fluoride Exporter (Fluc) Family	0.24	0.8	1	0.58	0.16	1	1	0.72	0.33	1	1	0.39	0.92	0.33	0.94	5e-04	0.00031	0.00018	0.00039	0.00022	0.00038	0.00019	0	0.00025	-1	11 / 18 (61%)	8 / 11 (73%)	3 / 7 (43%)	0.727	0.429
2.A.3.1.7	The Amino Acid-Polyamine-Organocation (APC) Family	0.29	0.8	1	-0.7	0.22	1	1	-0.78	0.53	1	1	0.43	0.93	0.49	0.95	0.00033	5.4e-05	0	5.4e-06	0	1.8e-05	0.00013	0	3e-04	4.6	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
3.A.1.147.4	The ATP-binding Cassette (ABC) Superfamily	0.17	0.8	1	-0.93	0.41	1	1	-0.52	0.33	1	1	0.5	0.93	0.46	0.95	0.00027	7.6e-05	0	9.5e-06	0	2.3e-05	0.00018	0	0.00032	4.2	5 / 18 (28%)	2 / 11 (18%)	3 / 7 (43%)	0.182	0.429
3.A.1.122.30	The ATP-binding Cassette (ABC) Superfamily	0.54	0.8	1	0.33	0.21	1	1	0.71	0.25	1	1	0.27	0.91	0.34	0.94	0.0024	0.0019	0.0017	0.002	0.0014	0.0017	0.0017	0.0019	0.0019	-0.23	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
3.A.1.6.1	The ATP-binding Cassette (ABC) Superfamily	0.31	0.8	1	-0.67	0.22	1	1	-0.77	0.53	1	1	0.44	0.92	0.45	0.94	0.00074	0.00012	0	8.1e-06	0	2.7e-05	0.00031	0	0.00076	5.3	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
1.A.43.3.1	The Camphor Resistance or Fluoride Exporter (Fluc) Family	0.67	0.81	1	0.24	0.28	1	1	0.6	0.25	1	1	0.43	0.93	0.46	0.95	0.00055	0.00043	0.00046	0.00042	4e-04	0.00032	0.00044	0.00059	0.00044	0.067	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
2.A.2.3.4	The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family	0.67	0.81	1	-0.26	0.55	1	1	0.27	1	1	1	0.48	0.93	0.51	0.95	0.00027	6.1e-05	0	2e-05	0	3.9e-05	0.00013	0	0.00033	2.7	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
2.A.17.1.6	The Proton-dependent Oligopeptide Transporter (POT/PTR) Family	0.21	0.8	1	-0.76	0.52	1	1	-0.38	1	1	1	0.53	0.94	0.51	0.95	0.00014	7.2e-05	1.5e-05	2.8e-05	0	3.9e-05	0.00014	6.8e-05	0.00018	2.3	9 / 18 (50%)	5 / 11 (45%)	4 / 7 (57%)	0.455	0.571
3.D.4.5.1	The Proton-translocating Cytochrome Oxidase (COX) Superfamily	0.36	0.8	1	-0.59	0.42	1	1	-0.47	1	1	1	0.48	0.93	0.49	0.94	5e-04	0.00011	0	1.6e-05	0	3.7e-05	0.00026	0	0.00063	4	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
2.A.1.7.17	The Major Facilitator Superfamily (MFS)	0.59	0.8	1	0.23	0.54	1	1	0.32	1	1	1	0.6	0.95	0.67	0.96	0.00031	8.7e-05	0	0.00012	0	0.00032	3.3e-05	0	5.6e-05	-1.9	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
9.B.211.1.2	The 10 TMS Putative Permease (10PP) Family	0.65	0.81	1	0.23	0.53	1	1	0.34	0.63	1	1	0.65	0.95	0.74	0.98	0.00023	0.00015	7.1e-05	0.00016	7.5e-05	2e-04	0.00013	6.2e-05	0.00017	-0.3	12 / 18 (67%)	8 / 11 (73%)	4 / 7 (57%)	0.727	0.571
5.A.3.4.1	The Prokaryotic Molybdopterin-containing Oxidoreductase (PMO) Family	0.4	0.8	1	-0.54	0.5	1	1	-0.37	1	1	1	0.5	0.93	0.55	0.95	0.00058	0.00013	0	2.6e-05	0	6.9e-05	0.00029	0	0.00071	3.5	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
5.A.3.4.3	The Prokaryotic Molybdopterin-containing Oxidoreductase (PMO) Family	0.62	0.8	1	-0.31	0.63	1	1	0.23	1	1	1	0.53	0.94	0.56	0.96	4e-04	8.9e-05	0	2.3e-05	0	4.1e-05	0.00019	0	0.00051	3	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
4.A.6.1.4	The PTS Mannose-Fructose-Sorbose (Man) Family	0.31	0.8	1	-0.63	0.37	1	1	-0.52	0.53	1	1	0.5	0.94	0.53	0.95	0.00024	4e-05	0	1.1e-05	0	3.6e-05	8.5e-05	0	0.00018	2.9	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
3.D.1.9.1	The H⁺ or Na⁺-translocating NADH Dehydrogenase (NDH) Family	0.58	0.8	1	-0.29	1	1	1	-0.0023	0.53	1	1	0.58	0.94	0.59	0.96	0.00016	2.6e-05	0	2.2e-05	0	7.1e-05	3.4e-05	0	7.9e-05	0.63	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
8.A.5.1.5	The Voltage-gated K⁺ Channel β-subunit (Kvβ) Family	0.55	0.8	1	-0.38	0.99	1	1	0.004	1	1	1	0.49	0.93	0.51	0.95	0.00034	5.7e-05	0	1.3e-05	0	3.5e-05	0.00013	0	0.00033	3.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.121.1.1	The Sulfate Transporter (CysZ) Family	0.6	0.8	1	-0.32	0.8	1	1	0.11	1	1	1	0.47	0.93	0.51	0.95	0.00033	5.5e-05	0	1.7e-05	0	3.9e-05	0.00012	0	0.00031	2.8	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.3.1.24	The Amino Acid-Polyamine-Organocation (APC) Family	0.75	0.85	1	-0.18	0.34	1	1	0.39	1	1	1	0.48	0.93	0.51	0.95	0.00017	2.8e-05	0	1.6e-05	0	3.7e-05	4.8e-05	0	0.00013	1.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.120.6	The ATP-binding Cassette (ABC) Superfamily	0.54	0.8	1	-0.38	1	1	1	-0.0017	1	1	1	0.51	0.93	0.57	0.96	0.00034	5.7e-05	0	1.3e-05	0	3.3e-05	0.00013	0	0.00033	3.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
9.B.34.1.2	The Kinase/Phosphatase/Cyclic-GMP Synthase/Cyclic di-GMP Hydrolase (KPSH) Family	0.49	0.8	1	-0.44	0.81	1	1	-0.12	1	1	1	0.52	0.94	0.56	0.96	0.00042	7e-05	0	9.5e-06	0	2.3e-05	0.00016	0	0.00044	4.1	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.17.3.10	The Outer Membrane Factor (OMF) Family	0.58	0.8	1	-0.34	0.86	1	1	0.08	1	1	1	0.51	0.93	0.54	0.95	0.00027	4.6e-05	0	1.3e-05	0	3.5e-05	9.7e-05	0	0.00026	2.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.1.2.65	The Major Facilitator Superfamily (MFS)	0.36	0.8	1	-0.57	0.51	1	1	-0.35	1	1	1	0.5	0.93	0.55	0.96	0.00015	3.4e-05	0	9.5e-06	0	2.3e-05	7.3e-05	0	0.00015	2.9	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
3.A.1.105.15	The ATP-binding Cassette (ABC) Superfamily	0.28	0.8	1	-0.72	0.24	1	1	-0.74	0.53	1	1	0.5	0.93	0.52	0.95	0.00033	5.5e-05	0	5.4e-06	0	1.8e-05	0.00013	0	3e-04	4.6	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
5.A.3.4.2	The Prokaryotic Molybdopterin-containing Oxidoreductase (PMO) Family	0.55	0.8	1	-0.37	0.93	1	1	0.044	1	1	1	0.5	0.94	0.55	0.96	0.00057	9.6e-05	0	2.1e-05	0	4.7e-05	0.00021	0	0.00056	3.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
4.A.1.1.3	The PTS Glucose-Glucoside (Glc) Family	0.81	0.88	1	-0.13	0.47	1	1	0.3	1	1	1	0.46	0.93	0.52	0.95	0.00031	5.2e-05	0	3.6e-05	0	1e-04	7.7e-05	0	2e-04	1.1	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.21.1	The ATP-binding Cassette (ABC) Superfamily	0.5	0.8	1	-0.43	0.83	1	1	-0.11	1	1	1	0.52	0.94	0.52	0.95	0.00052	8.7e-05	0	1.3e-05	0	3.5e-05	2e-04	0	0.00054	3.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.1.3.17	The Major Facilitator Superfamily (MFS)	0.6	0.8	1	-0.32	0.76	1	1	0.14	1	1	1	0.48	0.93	0.54	0.96	0.00031	5.1e-05	0	1.6e-05	0	3.7e-05	0.00011	0	0.00028	2.8	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.A.16.3.1	The Formate-Nitrite Transporter (FNT) Family	0.61	0.8	1	-0.31	0.73	1	1	0.15	1	1	1	0.49	0.93	0.53	0.95	0.00023	3.8e-05	0	1.3e-05	0	3.5e-05	7.7e-05	0	2e-04	2.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.1.1.1	The General Bacterial Porin (GBP) Family	0.78	0.88	1	-0.16	0.35	1	1	0.39	1	1	1	0.51	0.94	0.55	0.96	0.00021	3.5e-05	0	2.1e-05	0	4.7e-05	5.8e-05	0	0.00015	1.5	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.2.2	The ATP-binding Cassette (ABC) Superfamily	0.6	0.8	1	-0.32	0.81	1	1	0.11	1	1	1	0.49	0.93	0.51	0.95	0.00058	9.7e-05	0	2.9e-05	0	6.8e-05	2e-04	0	0.00054	2.8	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.2.1.1	The H⁺- or Na⁺-translocating F-type. V-type and A-type ATPase (F-ATPase) Superfamily	0.43	0.8	1	-0.51	0.67	1	1	-0.24	1	1	1	0.52	0.93	0.55	0.96	0.00087	0.00024	0	3.1e-05	0	5.5e-05	0.00058	0	0.0015	4.2	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
3.A.1.3.26	The ATP-binding Cassette (ABC) Superfamily	0.32	0.8	1	-0.65	0.24	1	1	-0.72	0.53	1	1	0.5	0.94	0.49	0.95	0.00077	0.00013	0	1.1e-05	0	3.6e-05	0.00032	0	0.00079	4.9	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.1.33.2	The Major Facilitator Superfamily (MFS)	0.93	0.96	1	-0.046	0.81	1	1	0.13	1	1	1	0.63	0.95	0.64	0.97	0.00041	0.00011	0	0.00011	0	0.00029	0.00012	0	0.00028	0.13	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
1.A.23.4.5	The Small Conductance Mechanosensitive Ion Channel (MscS) Family	0.52	0.8	1	-0.38	0.9	1	1	-0.053	1	1	1	0.48	0.93	0.55	0.96	0.00021	4.7e-05	0	1.8e-05	0	4.5e-05	9.1e-05	0	0.00023	2.3	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
3.A.1.25.6	The ATP-binding Cassette (ABC) Superfamily	0.69	0.83	1	-0.22	0.86	1	1	0.098	1	1	1	0.71	0.96	0.79	0.98	0.00051	0.00031	0.00023	0.00022	0.00022	0.00022	0.00046	0.00024	8e-04	1.1	11 / 18 (61%)	7 / 11 (64%)	4 / 7 (57%)	0.636	0.571
3.A.1.129.1	The ATP-binding Cassette (ABC) Superfamily	0.48	0.8	1	-0.45	0.82	1	1	-0.12	1	1	1	0.52	0.93	0.55	0.96	0.00041	0.00012	0	2e-05	0	3.9e-05	0.00027	0	0.00069	3.8	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
2.A.1.2.60	The Major Facilitator Superfamily (MFS)	0.25	0.8	1	-0.76	0.21	1	1	-0.83	0.53	1	1	0.48	0.92	0.52	0.94	2e-04	3.4e-05	0	2.7e-06	0	8.9e-06	8.3e-05	0	0.00018	4.9	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
9.B.226.1.8	The Uncharacterized 9 or 10 TMS Protein (U-TMP) Family	0.61	0.8	1	0.27	0.31	1	1	0.55	0.25	1	1	0.42	0.93	0.44	0.95	0.00098	0.00076	7e-04	0.00082	0.00044	0.00079	0.00068	0.00096	0.00065	-0.27	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
9.B.22.1.8	The Leukotoxin Secretion Morphogenesis Protein C (MorC) Family	0.37	0.8	1	-0.49	0.51	1	1	-0.36	0.33	1	1	0.56	0.94	0.61	0.97	0.0011	0.00031	0	0.00019	0	0.00053	0.00049	0	0.00089	1.4	5 / 18 (28%)	2 / 11 (18%)	3 / 7 (43%)	0.182	0.429
1.B.75.1.3	The DUF481 Putative Beta Barrel Porin (DUF481) Family	0.25	0.8	1	-0.77	0.21	1	1	-0.83	0.53	1	1	0.47	0.92	0.53	0.94	0.00023	3.8e-05	0	2.7e-06	0	8.9e-06	9.4e-05	0	2e-04	5.1	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.40.4.3	The Nucleobase/Ascorbate Transporter (NAT) or Nucleobase:Cation Symporter-2 (NCS2) Family	0.28	0.8	1	-0.69	0.35	1	1	-0.53	0.53	1	1	0.41	0.92	0.45	0.95	0.00027	4.4e-05	0	1e-05	0	3.4e-05	9.8e-05	0	0.00018	3.3	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.1.4.3	The Major Facilitator Superfamily (MFS)	0.55	0.8	1	-0.38	0.96	1	1	0.022	1	1	1	0.5	0.94	0.55	0.96	0.00034	5.6e-05	0	1.2e-05	0	2.6e-05	0.00013	0	0.00033	3.4	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
5.A.3.1.1	The Prokaryotic Molybdopterin-containing Oxidoreductase (PMO) Family	0.27	0.8	1	-0.74	0.18	1	1	-0.92	0.53	1	1	0.39	0.9	0.39	0.93	0.00055	9.1e-05	0	2.7e-06	0	8.9e-06	0.00023	0	0.00056	6.4	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.17.1.2	The Proton-dependent Oligopeptide Transporter (POT/PTR) Family	0.8	0.88	1	-0.14	0.25	1	1	0.46	1	1	1	0.5	0.93	0.53	0.95	0.00015	2.5e-05	0	1.6e-05	0	3.7e-05	3.9e-05	0	1e-04	1.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.125.1	The ATP-binding Cassette (ABC) Superfamily	0.58	0.8	1	-0.34	0.85	1	1	0.088	1	1	1	0.53	0.93	0.54	0.96	0.00038	6.3e-05	0	1.7e-05	0	4e-05	0.00014	0	0.00036	3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.5.42	The ATP-binding Cassette (ABC) Superfamily	0.38	0.8	1	-0.57	0.33	1	1	-0.53	0.53	1	1	0.49	0.93	0.52	0.95	0.00045	7.6e-05	0	1.1e-05	0	3.6e-05	0.00018	0	0.00046	4	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
9.B.186.1.1	The Putative Lipoprotein Supressor of a ts bamD mutant. YiaD (YiaD) Family	0.33	0.8	1	-0.59	0.49	1	1	-0.37	0.53	1	1	0.53	0.94	0.58	0.96	0.00016	2.7e-05	0	1e-05	0	3.4e-05	5.4e-05	0	1e-04	2.4	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.C.1.4.3	The TonB-ExbB-ExbD/TolA-TolQ-TolR Outer Membrane Receptor Energizers and Stabilizers (TonB/TolA) Family	0.1	0.8	1	0.68	0.082	1	1	0.89	0.25	1	1	0.38	0.92	0.4	0.94	0.00024	4e-05	0	6.5e-05	0	0.00014	0	0	0	-Inf	3 / 18 (17%)	3 / 11 (27%)	0 / 7 (0%)	0.273	0
1.A.1.5.25	The Voltage-gated Ion Channel (VIC) Superfamily	0.38	0.8	1	0.48	0.18	1	1	0.74	0.25	1	1	0.32	0.91	0.31	0.94	0.00068	0.00053	5e-04	0.00059	0.00057	0.00045	0.00044	0.00036	0.00046	-0.42	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
3.A.1.27.3	The ATP-binding Cassette (ABC) Superfamily	0.31	0.8	1	-0.67	0.27	1	1	-0.67	0.53	1	1	0.39	0.92	0.41	0.94	0.0011	0.00018	0	1.9e-05	0	6.2e-05	0.00044	0	0.0011	4.5	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.47.3.2	The Divalent Anion:Na⁺ Symporter (DASS) Family	0.7	0.83	1	-0.22	0.61	1	1	0.21	1	1	1	0.52	0.93	0.54	0.95	0.00027	4.4e-05	0	2.3e-05	0	6.8e-05	7.7e-05	0	2e-04	1.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
9.B.118.1.1	The 11 or 12 TMS YhfT (YhfT) Family	0.6	0.8	1	-0.32	0.76	1	1	0.14	1	1	1	0.46	0.93	0.5	0.95	0.00031	5.1e-05	0	1.6e-05	0	3.7e-05	0.00011	0	0.00028	2.8	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
4.A.6.1.7	The PTS Mannose-Fructose-Sorbose (Man) Family	0.42	0.8	1	-0.41	0.71	1	1	-0.19	1	1	1	0.67	0.96	0.75	0.97	0.00026	0.00021	0.00012	0.00017	8.8e-05	0.00021	0.00026	0.00013	0.00029	0.61	14 / 18 (78%)	8 / 11 (73%)	6 / 7 (86%)	0.727	0.857
2.A.1.7.18	The Major Facilitator Superfamily (MFS)	0.85	0.92	1	-0.092	0.87	1	1	0.089	1	1	1	0.64	0.95	0.67	0.97	0.00025	0.00013	1.3e-05	0.00012	0	0.00029	0.00013	2.7e-05	0.00024	0.12	9 / 18 (50%)	5 / 11 (45%)	4 / 7 (57%)	0.455	0.571
2.A.8.1.4	The Gluconate:H⁺ Symporter (GntP) Family	0.92	0.96	1	-0.057	0.18	1	1	0.59	0.6	1	1	0.51	0.93	0.54	0.96	0.00025	6.9e-05	0	3.9e-05	0	7e-05	0.00012	0	0.00031	1.6	5 / 18 (28%)	4 / 11 (36%)	1 / 7 (14%)	0.364	0.143
2.A.69.4.6	The Auxin Efflux Carrier (AEC) Family	0.44	0.8	1	0.41	0.2	1	1	0.71	0.25	1	1	0.33	0.91	0.32	0.94	0.001	0.00081	0.00078	0.00089	0.00091	0.00075	0.00068	0.00036	0.00076	-0.39	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
3.A.1.23.1	The ATP-binding Cassette (ABC) Superfamily	0.33	0.8	1	-0.55	0.68	1	1	-0.23	1	1	1	0.56	0.94	0.62	0.96	0.00028	0.00015	3.1e-05	9.1e-05	2.9e-05	0.00013	0.00025	0.00014	3e-04	1.5	10 / 18 (56%)	6 / 11 (55%)	4 / 7 (57%)	0.545	0.571
9.B.157.1.3	The Cell Shape-determining MreBCD (MreBCD) Family	0.53	0.8	1	-0.4	0.94	1	1	-0.036	1	1	1	0.51	0.93	0.54	0.95	0.00046	7.7e-05	0	1.6e-05	0	4.3e-05	0.00017	0	0.00046	3.4	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.40.3.3	The Nucleobase/Ascorbate Transporter (NAT) or Nucleobase:Cation Symporter-2 (NCS2) Family	0.31	0.8	1	-0.66	0.27	1	1	-0.63	0.53	1	1	0.45	0.92	0.5	0.94	0.00048	8.1e-05	0	1e-05	0	3.4e-05	0.00019	0	0.00046	4.2	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
1.B.33.1.3	The Outer Membrane Protein Insertion Porin (Bam Complex) (OmpIP) Family	0.51	0.8	1	-0.43	0.94	1	1	0.036	0.64	1	1	0.52	0.93	0.58	0.96	0.00064	0.00025	0	3.9e-05	0	5.3e-05	0.00058	0	0.0015	3.9	7 / 18 (39%)	5 / 11 (45%)	2 / 7 (29%)	0.455	0.286
3.A.1.17.1	The ATP-binding Cassette (ABC) Superfamily	0.66	0.81	1	-0.26	0.55	1	1	0.25	1	1	1	0.48	0.93	0.54	0.95	0.00018	3.1e-05	0	1.3e-05	0	3.5e-05	5.8e-05	0	0.00015	2.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.6.2.1	The Resistance-Nodulation-Cell Division (RND) Superfamily	0.5	0.8	1	-0.43	0.88	1	1	-0.077	1	1	1	0.5	0.93	0.49	0.95	0.00084	0.00014	0	1.8e-05	0	4.1e-05	0.00033	0	0.00087	4.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.55.3.1	The Poly Acetyl Glucosamine Porin (PgaA) Family	0.52	0.8	1	-0.41	0.95	1	1	-0.033	1	1	1	0.5	0.93	0.53	0.96	0.00068	0.00011	0	1.8e-05	0	4.1e-05	0.00026	0	0.00069	3.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.14.1.4	The Outer Membrane Receptor (OMR) Family	0.57	0.8	1	-0.35	0.88	1	1	0.07	1	1	1	0.5	0.93	0.55	0.96	0.00037	6.2e-05	0	1.6e-05	0	3.7e-05	0.00014	0	0.00036	3.1	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.27.1.1	The Glutamate:Na⁺ Symporter (ESS) Family	0.53	0.8	1	-0.39	0.99	1	1	-0.0065	1	1	1	0.5	0.94	0.53	0.95	0.00049	8.1e-05	0	1.6e-05	0	3.7e-05	0.00018	0	0.00049	3.5	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.15.5	The ATP-binding Cassette (ABC) Superfamily	0.53	0.8	1	-0.39	0.99	1	1	-0.0034	1	1	1	0.5	0.93	0.53	0.95	0.00046	7.7e-05	0	1.5e-05	0	3.3e-05	0.00017	0	0.00046	3.5	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
8.A.3.2.3	The Cytoplasmic Membrane-Periplasmic Auxiliary-1 (MPA1) Protein with Cytoplasmic (C) Domain (MPA1-C or MPA1+C) Family	0.3	0.8	1	-0.59	0.72	1	1	-0.21	0.63	1	1	0.61	0.95	0.54	0.96	0.00013	6e-05	0	3.4e-05	0	5.1e-05	1e-04	2.7e-05	0.00013	1.6	8 / 18 (44%)	4 / 11 (36%)	4 / 7 (57%)	0.364	0.571
3.A.1.2.3	The ATP-binding Cassette (ABC) Superfamily	0.47	0.8	1	-0.47	0.71	1	1	-0.19	1	1	1	0.51	0.91	0.55	0.95	0.00058	9.6e-05	0	9.2e-06	0	2.2e-05	0.00023	0	0.00061	4.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.3.2.8	The Amino Acid-Polyamine-Organocation (APC) Family	0.26	0.8	1	-0.76	0.2	1	1	-0.87	0.53	1	1	0.48	0.93	0.5	0.94	0.00032	5.4e-05	0	2.7e-06	0	8.9e-06	0.00013	0	3e-04	5.6	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.17.1.4	The Proton-dependent Oligopeptide Transporter (POT/PTR) Family	0.56	0.8	1	-0.37	0.85	1	1	0.092	1	1	1	0.49	0.93	0.54	0.95	0.00049	0.00011	0	1.9e-05	0	3.8e-05	0.00025	0	0.00067	3.7	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
2.A.115.2.9	The Novobiocin Exporter (NbcE) Family	0.25	0.8	1	0.48	0.22	1	1	0.63	1	1	1	0.51	0.94	0.53	0.96	0.00037	8.2e-05	0	0.00013	0	0.00028	1.3e-05	0	3.5e-05	-3.3	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
1.B.23.1.16	The Cyanobacterial Porin (CBP) Family	0.37	0.8	1	-0.55	0.55	1	1	-0.32	1	1	1	0.53	0.93	0.59	0.96	0.00026	5.7e-05	0	1.8e-05	0	4.5e-05	0.00012	0	0.00023	2.7	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
2.A.7.3.57	The Drug/Metabolite Transporter (DMT) Superfamily	0.79	0.88	1	0.12	0.71	1	1	0.2	1	1	1	0.59	0.94	0.63	0.96	0.00037	1e-04	0	0.00013	0	0.00039	5.3e-05	0	9.8e-05	-1.3	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
2.A.1.6.4	The Major Facilitator Superfamily (MFS)	0.7	0.83	1	-0.22	0.7	1	1	0.17	1	1	1	0.5	0.93	0.56	0.95	0.00037	6.2e-05	0	3.4e-05	0	1e-04	0.00011	0	0.00028	1.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.10.1.1	The Nucleoside-specific Channel-forming Outer Membrane Porin (Tsx) Family	0.61	0.8	1	-0.31	0.73	1	1	0.15	1	1	1	0.45	0.93	0.5	0.95	0.00023	3.8e-05	0	1.3e-05	0	3.5e-05	7.7e-05	0	2e-04	2.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.14.7.1	The Outer Membrane Receptor (OMR) Family	0.38	0.8	1	-0.56	0.44	1	1	-0.44	1	1	1	0.52	0.93	0.53	0.95	0.00081	0.00018	0	2.1e-05	0	4.7e-05	0.00043	0	0.0011	4.4	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
9.B.14.1.17	The Putative Heme Handling Protein (HHP) Family	0.59	0.8	1	0.24	0.54	1	1	0.32	1	1	1	0.66	0.95	0.72	0.97	0.00032	0.00016	2.7e-05	0.00021	5.9e-05	0.00057	8.1e-05	0	0.00014	-1.4	9 / 18 (50%)	6 / 11 (55%)	3 / 7 (43%)	0.545	0.429
3.A.1.121.6	The ATP-binding Cassette (ABC) Superfamily	0.45	0.8	1	0.35	0.41	1	1	0.43	0.64	1	1	0.53	0.93	0.54	0.96	0.00023	9.1e-05	0	0.00012	0	0.00022	4.8e-05	0	8.6e-05	-1.3	7 / 18 (39%)	5 / 11 (45%)	2 / 7 (29%)	0.455	0.286
1.B.25.1.13	The Outer Membrane Porin (Opr) Family	0.66	0.81	1	-0.26	0.55	1	1	0.25	1	1	1	0.54	0.94	0.55	0.96	0.00018	3.1e-05	0	1.3e-05	0	3.5e-05	5.8e-05	0	0.00015	2.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
9.B.223.1.4	The 4 TMS DUF2979 or YoaS (YoaS) Family	0.75	0.85	1	-0.15	0.98	1	1	-0.016	0.63	1	1	0.55	0.94	0.62	0.97	2e-04	6.6e-05	0	6.9e-05	0	2e-04	6.1e-05	0	0.00011	-0.18	6 / 18 (33%)	3 / 11 (27%)	3 / 7 (43%)	0.273	0.429
2.A.21.2.1	The Solute:Sodium Symporter (SSS) Family	0.32	0.8	1	-0.65	0.18	1	1	-0.84	0.53	1	1	0.5	0.93	0.53	0.95	0.00018	2.9e-05	0	2.7e-06	0	8.9e-06	7.2e-05	0	0.00018	4.7	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
3.D.2.1.1	The Proton-translocating Transhydrogenase (PTH) Family	0.5	0.8	1	-0.41	0.83	1	1	-0.11	1	1	1	0.51	0.93	0.55	0.96	0.00034	9.4e-05	0	3.3e-05	0	7e-05	0.00019	0	0.00046	2.5	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
9.B.126.1.2	The Putative Lipid IV Exporter (YhjD) Family	0.3	0.8	1	0.5	0.23	1	1	0.59	1	1	1	0.43	0.93	0.31	0.94	0.00062	0.00048	0.00035	0.00059	0.00038	0.00057	0.00032	0.00024	0.00034	-0.88	14 / 18 (78%)	9 / 11 (82%)	5 / 7 (71%)	0.818	0.714
3.A.1.12.15	The ATP-binding Cassette (ABC) Superfamily	0.76	0.87	1	-0.18	0.34	1	1	0.42	1	1	1	0.48	0.93	0.49	0.94	0.00026	5.7e-05	0	2.5e-05	0	4.4e-05	0.00011	0	0.00028	2.1	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
3.A.3.7.1	The P-type ATPase (P-ATPase) Superfamily	0.63	0.8	1	-0.29	0.75	1	1	0.14	1	1	1	0.47	0.93	0.49	0.95	0.00041	6.9e-05	0	2.6e-05	0	6.9e-05	0.00014	0	0.00036	2.4	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.5.10	The ATP-binding Cassette (ABC) Superfamily	0.9	0.95	1	0.073	0.45	1	1	0.43	0.32	1	1	0.62	0.95	0.56	0.96	0.00027	8.9e-05	0	6e-05	0	8.2e-05	0.00013	0	0.00036	1.1	6 / 18 (33%)	5 / 11 (45%)	1 / 7 (14%)	0.455	0.143
2.A.1.42.1	The Major Facilitator Superfamily (MFS)	0.59	0.8	1	-0.33	0.8	1	1	0.11	1	1	1	0.46	0.93	0.5	0.95	0.00025	4.2e-05	0	1.3e-05	0	3.5e-05	8.7e-05	0	0.00023	2.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.4.10	The ATP-binding Cassette (ABC) Superfamily	0.67	0.81	1	0.21	0.2	1	1	0.52	1	1	1	0.49	0.93	0.52	0.95	0.00017	3.8e-05	0	4.3e-05	0	8.9e-05	2.9e-05	0	7.7e-05	-0.57	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
9.B.114.1.1	The Vancomycin-sensitivity protein (SanA) Family	0.82	0.9	1	-0.12	0.44	1	1	0.31	1	1	1	0.49	0.93	0.53	0.96	0.00023	3.9e-05	0	2.7e-05	0	7e-05	5.8e-05	0	0.00015	1.1	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.6.2.2	The Resistance-Nodulation-Cell Division (RND) Superfamily	0.36	0.8	1	-0.6	0.26	1	1	-0.67	0.53	1	1	0.45	0.93	0.47	0.95	0.00067	0.00011	0	8.1e-06	0	2.7e-05	0.00028	0	0.00072	5.1	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.6.2.12	The Resistance-Nodulation-Cell Division (RND) Superfamily	0.47	0.8	1	-0.45	0.69	1	1	-0.2	1	1	1	0.53	0.94	0.56	0.95	0.00065	0.00014	0	3.4e-05	0	7.8e-05	0.00032	0	0.00082	3.2	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
2.A.3.1.6	The Amino Acid-Polyamine-Organocation (APC) Family	0.58	0.8	1	-0.34	0.86	1	1	0.08	1	1	1	0.45	0.93	0.47	0.95	0.00027	4.6e-05	0	1.3e-05	0	3.5e-05	9.7e-05	0	0.00026	2.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.9.3.1	The Membrane Protein Insertase (YidC/Alb3/Oxa1) Family	0.66	0.81	1	-0.26	0.68	1	1	0.18	1	1	1	0.51	0.94	0.53	0.96	0.00037	6.1e-05	0	2.6e-05	0	6.9e-05	0.00012	0	0.00031	2.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.1.48	The ATP-binding Cassette (ABC) Superfamily	0.88	0.94	1	0.072	0.66	1	1	0.21	1	1	1	0.65	0.95	0.68	0.97	0.0026	0.0013	5e-05	0.0014	1e-04	0.0021	0.0012	0	0.0017	-0.22	9 / 18 (50%)	6 / 11 (55%)	3 / 7 (43%)	0.545	0.429
2.A.6.1.4	The Resistance-Nodulation-Cell Division (RND) Superfamily	0.74	0.85	1	-0.19	0.38	1	1	0.39	1	1	1	0.49	0.93	0.54	0.95	3e-04	6.6e-05	0	2.8e-05	0	4.9e-05	0.00013	0	0.00033	2.2	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
2.A.15.2.1	The Betaine/Carnitine/Choline Transporter (BCCT) Family	0.5	0.8	1	-0.42	0.85	1	1	-0.1	1	1	1	0.47	0.92	0.5	0.95	5e-04	8.3e-05	0	1.3e-05	0	3.5e-05	0.00019	0	0.00051	3.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.A.8.3.1	The Major Intrinsic Protein (MIP) Family	0.91	0.96	1	-0.057	0.23	1	1	0.49	1	1	1	0.5	0.93	0.56	0.96	0.00013	2.2e-05	0	1.8e-05	0	4e-05	2.9e-05	0	7.7e-05	0.69	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.81.1.5	The Aspartate:Alanine Exchanger (AAEx) Family	0.53	0.8	1	-0.39	0.99	1	1	0.0083	1	1	1	0.5	0.93	0.54	0.96	0.00066	0.00011	0	2.1e-05	0	4.7e-05	0.00025	0	0.00067	3.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.66.2.7	The Multidrug/Oligosaccharidyl-lipid/Polysaccharide (MOP) Flippase Superfamily	0.39	0.8	1	-0.54	0.39	1	1	-0.43	0.53	1	1	0.45	0.93	0.49	0.95	0.00027	4.6e-05	0	1.1e-05	0	3.6e-05	1e-04	0	0.00025	3.2	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
4.A.6.1.1	The PTS Mannose-Fructose-Sorbose (Man) Family	0.44	0.8	1	-0.47	0.63	1	1	-0.25	1	1	1	0.47	0.93	0.48	0.95	0.00024	5.4e-05	0	1.6e-05	0	3.7e-05	0.00011	0	0.00028	2.8	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
1.B.54.1.9	The Intimin/Invasin (Int/Inv) or Autotransporter-3 (AT-3) Family	0.27	0.8	1	-0.72	0.24	1	1	-0.76	0.53	1	1	0.46	0.93	0.5	0.95	0.00014	2.4e-05	0	2.7e-06	0	8.9e-06	5.7e-05	0	0.00013	4.4	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
3.A.1.5.21	The ATP-binding Cassette (ABC) Superfamily	0.36	0.8	1	-0.6	0.28	1	1	-0.66	0.53	1	1	0.43	0.92	0.45	0.94	0.00083	0.00014	0	8.1e-06	0	2.7e-05	0.00034	0	0.00089	5.4	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
3.A.1.8.1	The ATP-binding Cassette (ABC) Superfamily	0.46	0.8	1	-0.48	0.68	1	1	-0.22	1	1	1	0.52	0.93	0.53	0.94	0.00069	0.00011	0	9.2e-06	0	2.2e-05	0.00028	0	0.00074	4.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.1.7.14	The Major Facilitator Superfamily (MFS)	0.27	0.8	1	-0.74	0.17	1	1	-0.94	0.53	1	1	0.42	0.91	0.46	0.93	0.00036	6e-05	0	2.7e-06	0	8.9e-06	0.00015	0	0.00035	5.8	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
1.B.3.1.1	The Sugar Porin (SP) Family	0.55	0.8	1	-0.38	0.99	1	1	0.004	1	1	1	0.46	0.92	0.52	0.94	0.00034	5.7e-05	0	1.3e-05	0	3.5e-05	0.00013	0	0.00033	3.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.14.3	The ATP-binding Cassette (ABC) Superfamily	0.82	0.9	1	-0.12	0.52	1	1	0.27	1	1	1	0.53	0.94	0.56	0.96	0.00062	1e-04	0	7e-05	0	0.00018	0.00015	0	0.00041	1.1	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
4.A.1.2.3	The PTS Glucose-Glucoside (Glc) Family	0.26	0.8	1	-0.76	0.2	1	1	-0.86	0.53	1	1	0.5	0.94	0.53	0.96	0.00023	3.8e-05	0	2.7e-06	0	8.9e-06	9.3e-05	0	2e-04	5.1	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.C.1.4.2	The TonB-ExbB-ExbD/TolA-TolQ-TolR Outer Membrane Receptor Energizers and Stabilizers (TonB/TolA) Family	0.2	0.8	1	-0.82	0.2	1	1	-0.74	0.047	1	1	0.33	0.91	0.33	0.93	0.001	0.00028	0	6.3e-05	0	0.00021	0.00063	2.7e-05	0.0013	3.3	5 / 18 (28%)	1 / 11 (9.1%)	4 / 7 (57%)	0.0909	0.571
1.B.14.6.1	The Outer Membrane Receptor (OMR) Family	0.36	0.8	1	0.43	0.3	1	1	0.58	0.6	1	1	0.5	0.93	0.52	0.95	8.7e-05	2.4e-05	0	3.1e-05	0	5.1e-05	1.3e-05	0	3.5e-05	-1.3	5 / 18 (28%)	4 / 11 (36%)	1 / 7 (14%)	0.364	0.143
1.A.23.1.3	The Small Conductance Mechanosensitive Ion Channel (MscS) Family	0.56	0.8	1	-0.37	0.83	1	1	0.11	1	1	1	0.5	0.93	0.55	0.95	0.00051	0.00011	0	1.9e-05	0	3.3e-05	0.00026	0	0.00069	3.8	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
1.B.71.1.5	The Proteobacterial/Verrucomicrobial Porin (PVP) Family	0.78	0.88	1	0.14	0.19	1	1	0.54	1	1	1	0.52	0.94	0.54	0.95	3e-04	6.7e-05	0	6.7e-05	0	0.00012	6.8e-05	0	0.00018	0.021	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
2.A.37.1.2	The Monovalent Cation:Proton Antiporter-2 (CPA2) Family	0.63	0.8	1	-0.29	0.63	1	1	0.2	1	1	1	0.53	0.94	0.57	0.96	2e-04	3.4e-05	0	1.2e-05	0	2.7e-05	6.8e-05	0	0.00018	2.5	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.152.9	The ATP-binding Cassette (ABC) Superfamily	0.5	0.8	1	-0.41	0.86	1	1	-0.087	1	1	1	0.52	0.94	0.56	0.96	0.00047	0.00013	0	3.8e-05	0	7.4e-05	0.00028	0	0.00069	2.9	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
2.A.76.1.1	The Resistance to Homoserine/Threonine (RhtB) Family	0.99	0.99	1	0.0064	0.2	1	1	0.53	1	1	1	0.47	0.93	0.52	0.94	0.00023	5e-05	0	3.9e-05	0	7.7e-05	6.8e-05	0	0.00018	0.8	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
4.A.1.1.1	The PTS Glucose-Glucoside (Glc) Family	0.59	0.8	1	-0.33	0.9	1	1	0.06	1	1	1	0.5	0.93	0.51	0.96	0.00042	7.1e-05	0	2.3e-05	0	6.8e-05	0.00015	0	0.00038	2.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.9.1	The ATP-binding Cassette (ABC) Superfamily	0.5	0.8	1	-0.43	0.82	1	1	-0.12	1	1	1	0.48	0.92	0.53	0.95	0.00054	9.1e-05	0	1.3e-05	0	3.5e-05	0.00021	0	0.00056	4	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.25.1.10	The Alanine or Glycine:Cation Symporter (AGCS) Family	0.62	0.8	1	-0.3	0.79	1	1	0.11	1	1	1	0.49	0.93	0.56	0.96	0.00026	4.3e-05	0	1.6e-05	0	4.3e-05	8.7e-05	0	0.00023	2.4	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.2.25	The ATP-binding Cassette (ABC) Superfamily	0.47	0.8	1	-0.47	0.79	1	1	-0.14	1	1	1	0.51	0.94	0.53	0.96	0.00096	0.00016	0	1.5e-05	0	3.3e-05	0.00039	0	0.001	4.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.D.1.1.1	The H⁺ or Na⁺-translocating NADH Dehydrogenase (NDH) Family	0.47	0.8	1	-0.46	0.79	1	1	-0.14	1	1	1	0.56	0.94	0.63	0.97	0.00099	0.00027	0	5.1e-05	0	0.00011	0.00062	0	0.0016	3.6	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
8.A.46.2.2	The Glycan-binding Protein (SusD) Family	0.97	0.98	1	0.019	0.64	1	1	0.27	1	1	1	0.57	0.94	0.61	0.96	0.00011	1.8e-05	0	1.8e-05	0	5e-05	1.8e-05	0	4.7e-05	0	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.1.2.19	The Major Facilitator Superfamily (MFS)	0.95	0.97	1	-0.035	0.26	1	1	0.46	1	1	1	0.48	0.93	0.52	0.96	0.00015	2.6e-05	0	2.3e-05	0	6.8e-05	2.9e-05	0	7.7e-05	0.33	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.1.21.1	The Major Facilitator Superfamily (MFS)	0.31	0.8	1	0.43	0.25	1	1	0.59	0.6	1	1	0.49	0.94	0.47	0.95	0.0012	0.00033	0	5e-04	0	0.0014	7.1e-05	0	0.00019	-2.8	5 / 18 (28%)	4 / 11 (36%)	1 / 7 (14%)	0.364	0.143
3.A.7.10.1	The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family	0.93	0.96	1	0.046	0.59	1	1	0.31	1	1	1	0.53	0.93	0.57	0.96	9.2e-05	1.5e-05	0	1.5e-05	0	3.6e-05	1.5e-05	0	4e-05	0	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.5.25	The ATP-binding Cassette (ABC) Superfamily	0.84	0.91	1	-0.098	0.79	1	1	0.13	1	1	1	0.55	0.94	0.63	0.96	0.00014	3.1e-05	0	3.3e-05	0	8.7e-05	2.9e-05	0	5.3e-05	-0.19	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
2.A.1.3.51	The Major Facilitator Superfamily (MFS)	0.58	0.8	1	-0.35	0.8	1	1	0.12	1	1	1	0.48	0.93	0.52	0.95	0.00049	0.00011	0	2.2e-05	0	4.6e-05	0.00024	0	0.00064	3.4	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
2.A.42.1.3	The Hydroxy/Aromatic Amino Acid Permease (HAAAP) Family	0.58	0.8	1	-0.34	0.86	1	1	0.08	1	1	1	0.52	0.94	0.57	0.96	0.00027	4.6e-05	0	1.3e-05	0	3.5e-05	9.7e-05	0	0.00026	2.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.3.32.7	The P-type ATPase (P-ATPase) Superfamily	0.6	0.8	1	-0.3	0.99	1	1	0.0037	1	1	1	0.53	0.94	0.56	0.96	0.00051	0.00014	0	7.4e-05	0	0.00016	0.00025	0	0.00058	1.8	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
2.A.118.1.8	The Basic Amino Acid Antiporter (ArcD) Family	0.95	0.97	1	-0.032	0.14	1	1	0.61	1	1	1	0.49	0.93	0.51	0.94	0.00015	3.3e-05	0	2.3e-05	0	4.1e-05	4.8e-05	0	0.00013	1.1	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
3.A.1.17.10	The ATP-binding Cassette (ABC) Superfamily	0.53	0.8	1	-0.4	0.94	1	1	-0.037	1	1	1	0.47	0.92	0.52	0.95	0.00031	5.1e-05	0	9.5e-06	0	2.3e-05	0.00012	0	0.00031	3.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.14.3.1	The Outer Membrane Receptor (OMR) Family	0.56	0.8	1	-0.36	0.98	1	1	0.0096	1	1	1	0.51	0.93	0.55	0.96	0.00051	8.6e-05	0	2.3e-05	0	6.8e-05	0.00018	0	0.00049	3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.46.1.9	The Benzoate:H+ Symporter (BenE) Family	0.66	0.81	1	-0.26	0.55	1	1	0.25	1	1	1	0.55	0.94	0.57	0.96	0.00018	3.1e-05	0	1.3e-05	0	3.5e-05	5.8e-05	0	0.00015	2.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.2.2.1	The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family	0.55	0.8	1	-0.31	0.99	1	1	0.0042	1	1	1	0.67	0.95	0.73	0.98	0.00018	0.00015	0.00012	0.00012	1e-04	0.00013	0.00019	0.00013	0.00018	0.66	15 / 18 (83%)	9 / 11 (82%)	6 / 7 (86%)	0.818	0.857
1.B.22.1.3	The Outer Bacterial Membrane Secretin (Secretin) Family	0.49	0.8	1	-0.44	0.84	1	1	-0.11	1	1	1	0.49	0.93	0.52	0.95	0.00076	0.00013	0	1.6e-05	0	3.7e-05	3e-04	0	0.00079	4.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.31.1	The ATP-binding Cassette (ABC) Superfamily	0.5	0.8	1	0.36	0.24	1	1	0.64	0.25	1	1	0.32	0.92	0.32	0.94	0.0017	0.0014	0.0011	0.0015	0.0012	0.0013	0.0012	0.00084	0.0013	-0.32	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
9.A.8.1.1	The Ferrous Iron Uptake (FeoB) Family	0.4	0.8	1	-0.53	0.51	1	1	-0.36	1	1	1	0.48	0.93	0.52	0.95	0.00046	1e-04	0	2.1e-05	0	4.7e-05	0.00023	0	0.00056	3.5	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
3.A.1.105.17	The ATP-binding Cassette (ABC) Superfamily	0.34	0.8	1	-0.62	0.33	1	1	-0.56	1	1	1	0.5	0.93	0.54	0.95	0.00041	9.1e-05	0	9.5e-06	0	2.3e-05	0.00022	0	0.00053	4.5	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
4.A.2.1.11	The PTS Fructose-Mannitol (Fru) Family	0.54	0.8	1	-0.38	0.97	1	1	0.018	1	1	1	0.51	0.93	0.53	0.95	0.00062	1e-04	0	2.3e-05	0	5.6e-05	0.00023	0	0.00061	3.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
4.A.2.1.2	The PTS Fructose-Mannitol (Fru) Family	0.53	0.8	1	-0.39	0.99	1	1	-0.0057	1	1	1	0.47	0.93	0.48	0.95	0.00038	6.4e-05	0	1.2e-05	0	2.7e-05	0.00015	0	0.00038	3.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.115.2.12	The Novobiocin Exporter (NbcE) Family	0.03	0.8	1	1.2	0.04	1	1	1.3	0.14	1	1	0.12	0.9	0.14	0.92	0.00061	0.00054	0.00028	0.00072	0.00097	5e-04	0.00026	0.00013	0.00031	-1.5	16 / 18 (89%)	11 / 11 (100%)	5 / 7 (71%)	1	0.714
2.A.7.3.12	The Drug/Metabolite Transporter (DMT) Superfamily	0.69	0.83	1	-0.23	0.59	1	1	0.24	1	1	1	0.45	0.93	0.5	0.95	0.00038	6.3e-05	0	2.9e-05	0	6.8e-05	0.00012	0	0.00031	2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
9.B.126.2.1	The Putative Lipid IV Exporter (YhjD) Family	0.56	0.8	1	-0.36	0.92	1	1	0.043	1	1	1	0.5	0.93	0.54	0.95	0.00034	5.7e-05	0	1.3e-05	0	3e-05	0.00013	0	0.00033	3.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
4.A.5.1.1	The PTS Galactitol (Gat) Family	0.15	0.8	1	-1	0.058	1	1	-1.4	0.043	1	1	0.31	0.88	0.33	0.91	0.00056	9.3e-05	0	0	0	0	0.00024	0	0.00053	Inf	3 / 18 (17%)	0 / 11 (0%)	3 / 7 (43%)	0	0.429
3.A.1.2.23	The ATP-binding Cassette (ABC) Superfamily	0.45	0.8	1	0.41	0.18	1	1	0.74	0.25	1	1	0.24	0.91	0.22	0.93	0.0039	0.003	0.0028	0.0033	0.0031	0.0025	0.0026	0.0022	0.0031	-0.34	14 / 18 (78%)	10 / 11 (91%)	4 / 7 (57%)	0.909	0.571
2.A.1.64.1	The Major Facilitator Superfamily (MFS)	0.12	0.8	1	-1	0.4	1	1	-0.53	0.14	1	1	0.44	0.93	0.33	0.94	0.00014	4.8e-05	0	9.5e-06	0	2.3e-05	0.00011	3.1e-05	0.00017	3.5	6 / 18 (33%)	2 / 11 (18%)	4 / 7 (57%)	0.182	0.571
2.A.10.1.2	The 2-Keto-3-Deoxygluconate Transporter (KdgT) Family	0.63	0.8	1	-0.29	0.63	1	1	0.2	1	1	1	0.49	0.93	0.51	0.95	2e-04	3.4e-05	0	1.2e-05	0	2.7e-05	6.8e-05	0	0.00018	2.5	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
4.C.1.1.4	The Fatty Acid Transporter (FAT) Family	0.26	0.8	1	-0.75	0.25	1	1	-0.66	0.53	1	1	0.43	0.91	0.46	0.94	0.00041	6.9e-05	0	6.8e-06	0	2.3e-05	0.00017	0	0.00035	4.6	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.5.5.5	The Zinc (Zn²⁺)-Iron (Fe²⁺) Permease (ZIP) Family	0.28	0.8	1	-0.61	0.42	1	1	-0.42	0.63	1	1	0.41	0.93	0.43	0.95	2e-04	8.8e-05	0	5e-05	0	8.8e-05	0.00015	8.1e-05	0.00021	1.6	8 / 18 (44%)	4 / 11 (36%)	4 / 7 (57%)	0.364	0.571
3.A.1.1.1	The ATP-binding Cassette (ABC) Superfamily	0.43	0.8	1	-0.49	0.6	1	1	-0.27	1	1	1	0.5	0.93	0.56	0.95	0.00069	0.00015	0	3.7e-05	0	8.3e-05	0.00034	0	0.00084	3.2	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
1.B.57.4.2	The Legionella Major-Outer Membrane Protein (LM-OMP) Family	0.97	0.98	1	-0.02	0.82	1	1	0.12	1	1	1	0.62	0.95	0.65	0.97	0.00023	5e-05	0	5.9e-05	0	0.00015	3.7e-05	0	6.5e-05	-0.67	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
2.A.75.1.2	The L-Lysine Exporter (LysE) Family	0.28	0.8	1	-0.71	0.23	1	1	-0.77	0.53	1	1	0.43	0.93	0.47	0.95	0.00033	5.5e-05	0	5.4e-06	0	1.8e-05	0.00013	0	3e-04	4.6	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
3.A.1.14.18	The ATP-binding Cassette (ABC) Superfamily	0.31	0.8	1	-0.66	0.27	1	1	-0.66	0.53	1	1	0.48	0.94	0.53	0.95	0.00049	8.1e-05	0	1.1e-05	0	3.6e-05	0.00019	0	0.00046	4.1	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.3.1.23	The Amino Acid-Polyamine-Organocation (APC) Family	0.58	0.8	1	-0.34	0.86	1	1	0.08	1	1	1	0.48	0.93	0.52	0.95	0.00027	4.6e-05	0	1.3e-05	0	3.5e-05	9.7e-05	0	0.00026	2.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.114.1.1	The Putative Peptide Transporter Carbon Starvation CstA (CstA) Family	0.39	0.8	1	-0.55	0.47	1	1	-0.4	1	1	1	0.51	0.94	0.53	0.96	0.00045	1e-04	0	1.8e-05	0	4.1e-05	0.00023	0	0.00056	3.7	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
2.A.1.1.115	The Major Facilitator Superfamily (MFS)	0.33	0.8	1	-0.6	0.43	1	1	-0.45	0.53	1	1	0.51	0.94	0.56	0.96	0.00019	3.1e-05	0	1.1e-05	0	3.6e-05	6.4e-05	0	0.00013	2.5	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.41.1.1	The Concentrative Nucleoside Transporter (CNT) Family	0.27	0.8	1	-0.74	0.17	1	1	-0.93	0.53	1	1	0.41	0.92	0.46	0.95	0.00034	5.6e-05	0	2.7e-06	0	8.9e-06	0.00014	0	0.00033	5.7	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
1.A.1.13.1	The Voltage-gated Ion Channel (VIC) Superfamily	0.57	0.8	1	-0.35	0.91	1	1	0.051	1	1	1	0.53	0.94	0.54	0.96	3e-04	4.9e-05	0	1.3e-05	0	3.5e-05	0.00011	0	0.00028	3.1	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.5.41	The ATP-binding Cassette (ABC) Superfamily	0.62	0.8	1	-0.32	0.54	1	1	0.31	0.6	1	1	0.51	0.93	0.59	0.96	8e-04	0.00022	0	4.5e-05	0	7.4e-05	5e-04	0	0.0013	3.5	5 / 18 (28%)	4 / 11 (36%)	1 / 7 (14%)	0.364	0.143
3.A.6.2.1	The Type III (Virulence-related) Secretory Pathway (IIISP) Family	0.53	0.8	1	-0.39	0.98	1	1	-0.014	1	1	1	0.5	0.93	0.51	0.96	0.00028	4.7e-05	0	9.5e-06	0	2.3e-05	0.00011	0	0.00028	3.5	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
9.A.69.1.1	The Intermembrane Phospholipid Translocase (IMPL-T) Family	0.49	0.8	1	-0.44	0.88	1	1	-0.082	1	1	1	0.52	0.94	0.55	0.96	0.00086	0.00014	0	1.8e-05	0	4.1e-05	0.00034	0	9e-04	4.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.21.7.2	The Solute:Sodium Symporter (SSS) Family	0.58	0.8	1	-0.34	0.86	1	1	0.08	1	1	1	0.5	0.94	0.55	0.96	0.00027	4.6e-05	0	1.3e-05	0	3.5e-05	9.7e-05	0	0.00026	2.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.2.22	The ATP-binding Cassette (ABC) Superfamily	0.36	0.8	1	0.47	0.24	1	1	0.61	0.53	1	1	0.31	0.91	0.29	0.94	0.0034	0.0029	0.0025	0.0033	0.0026	0.0026	0.0022	0.002	0.0022	-0.58	15 / 18 (83%)	10 / 11 (91%)	5 / 7 (71%)	0.909	0.714
2.A.15.1.4	The Betaine/Carnitine/Choline Transporter (BCCT) Family	0.51	0.8	1	-0.41	0.88	1	1	-0.078	1	1	1	0.49	0.93	0.5	0.95	0.00045	7.6e-05	0	1.3e-05	0	3.5e-05	0.00017	0	0.00046	3.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
8.A.4.1.1	The Cytoplasmic Membrane-Periplasmic Auxiliary-2 (MPA2) Family	0.56	0.8	1	-0.37	0.96	1	1	0.026	1	1	1	0.5	0.92	0.49	0.95	0.00032	5.3e-05	0	1.3e-05	0	3.5e-05	0.00012	0	0.00031	3.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.11.3.9	The Outer Membrane Fimbrial Usher Porin (FUP) Family	0.57	0.8	1	-0.35	0.89	1	1	0.067	1	1	1	0.5	0.93	0.59	0.96	0.00051	8.5e-05	0	2.2e-05	0	5e-05	0.00018	0	0.00049	3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
9.A.69.1.2	The Intermembrane Phospholipid Translocase (IMPL-T) Family	0.3	0.8	1	-0.68	0.25	1	1	-0.71	0.53	1	1	0.49	0.92	0.51	0.95	0.00043	7.2e-05	0	8.1e-06	0	2.7e-05	0.00017	0	4e-04	4.4	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.7.3.61	The Drug/Metabolite Transporter (DMT) Superfamily	0.66	0.81	1	-0.26	0.58	1	1	0.24	1	1	1	0.57	0.94	0.59	0.95	0.00027	4.5e-05	0	1.8e-05	0	4.1e-05	8.7e-05	0	0.00023	2.3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.12.1	The ATP-binding Cassette (ABC) Superfamily	0.53	0.8	1	-0.4	0.94	1	1	0.037	1	1	1	0.54	0.94	0.55	0.96	0.00052	0.00012	0	1.6e-05	0	3e-05	0.00027	0	0.00072	4.1	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
3.A.3.32.6	The P-type ATPase (P-ATPase) Superfamily	0.62	0.8	1	0.22	0.49	1	1	0.36	1	1	1	0.62	0.95	0.63	0.97	0.00031	5.1e-05	0	6.7e-05	0	0.00015	2.6e-05	0	7e-05	-1.4	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.127.1.2	The Enterobacterial Cardiolipin Transporter (CLT) Family	0.55	0.8	1	-0.37	0.94	1	1	0.035	1	1	1	0.54	0.94	0.56	0.96	0.00042	7e-05	0	1.6e-05	0	3.7e-05	0.00015	0	0.00041	3.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.A.77.3.5	The Mg²⁺/Ca²⁺ Uniporter (MCU) Family	0.2	0.8	1	-0.75	0.57	1	1	-0.34	0.33	1	1	0.5	0.94	0.38	0.94	0.00012	4.5e-05	0	2.2e-05	0	4.3e-05	8.1e-05	3.1e-05	1e-04	1.9	7 / 18 (39%)	3 / 11 (27%)	4 / 7 (57%)	0.273	0.571
1.B.1.1.3	The General Bacterial Porin (GBP) Family	0.61	0.8	1	-0.3	0.75	1	1	0.14	1	1	1	0.51	0.94	0.52	0.95	0.00023	3.8e-05	0	1.3e-05	0	3.5e-05	7.7e-05	0	2e-04	2.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.106.1	The ATP-binding Cassette (ABC) Superfamily	0.74	0.85	1	-0.19	0.45	1	1	0.32	1	1	1	0.53	0.94	0.57	0.96	0.00027	4.5e-05	0	2.4e-05	0	5.3e-05	7.7e-05	0	2e-04	1.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.85.1.2	The Aromatic Acid Exporter (ArAE) Family	0.53	0.8	1	-0.4	0.97	1	1	-0.018	1	1	1	0.46	0.92	0.51	0.95	0.00051	8.5e-05	0	1.6e-05	0	3.7e-05	0.00019	0	0.00051	3.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.1.140.1	The ATP-binding Cassette (ABC) Superfamily	0.29	0.8	1	-0.71	0.21	1	1	-0.82	0.53	1	1	0.48	0.93	0.52	0.95	0.00049	8.1e-05	0	5.4e-06	0	1.8e-05	2e-04	0	0.00048	5.2	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
3.A.1.106.20	The ATP-binding Cassette (ABC) Superfamily	0.74	0.85	1	-0.19	0.45	1	1	0.32	1	1	1	0.52	0.93	0.53	0.95	0.00027	4.5e-05	0	2.4e-05	0	5.3e-05	7.7e-05	0	2e-04	1.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.40.7.6	The Nucleobase/Ascorbate Transporter (NAT) or Nucleobase:Cation Symporter-2 (NCS2) Family	0.29	0.8	1	-0.7	0.2	1	1	-0.83	0.53	1	1	0.45	0.93	0.46	0.94	0.00048	8.1e-05	0	5.4e-06	0	1.8e-05	2e-04	0	0.00048	5.2	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
1.B.38.4.2	The Treponema Porin Major Surface Protein (TP-MSP) Family	0.52	0.8	1	0.28	0.38	1	1	0.5	1	1	1	0.53	0.94	0.57	0.96	7e-05	1.2e-05	0	1.6e-05	0	3.8e-05	4.4e-06	0	1.2e-05	-1.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.42.1.1	The Hydroxy/Aromatic Amino Acid Permease (HAAAP) Family	0.59	0.8	1	-0.33	0.8	1	1	0.11	1	1	1	0.48	0.93	0.52	0.96	0.00025	4.2e-05	0	1.3e-05	0	3.5e-05	8.7e-05	0	0.00023	2.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.B.14.8.7	The Outer Membrane Receptor (OMR) Family	0.59	0.8	1	-0.33	0.8	1	1	0.12	1	1	1	0.52	0.94	0.55	0.95	0.00038	6.4e-05	0	1.8e-05	0	4.1e-05	0.00014	0	0.00036	3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.D.1.9.2	The H⁺ or Na⁺-translocating NADH Dehydrogenase (NDH) Family	0.53	0.8	1	-0.39	0.99	1	1	0.0073	1	1	1	0.48	0.92	0.52	0.94	0.00084	0.00014	0	2.6e-05	0	6.1e-05	0.00032	0	0.00084	3.6	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
1.A.29.1.4	The Urea/Amide Channel (UAC) Family	0.084	0.8	1	0.79	0.12	1	1	0.8	0.37	1	1	0.43	0.93	0.36	0.94	0.00015	6.6e-05	0	9.6e-05	7.5e-05	0.00011	1.9e-05	0	4e-05	-2.3	8 / 18 (44%)	6 / 11 (55%)	2 / 7 (29%)	0.545	0.286
4.C.1.1.16	The Fatty Acid Transporter (FAT) Family	0.51	0.8	1	-0.41	0.88	1	1	-0.078	1	1	1	0.47	0.92	0.51	0.95	0.00045	7.6e-05	0	1.3e-05	0	3.5e-05	0.00017	0	0.00046	3.7	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.20.1.2	The Inorganic Phosphate Transporter (PiT) Family	0.27	0.8	1	-0.7	0.33	1	1	-0.53	0.53	1	1	0.47	0.93	0.51	0.95	0.00033	5.6e-05	0	1e-05	0	3.4e-05	0.00013	0	0.00026	3.7	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.3.7.5	The Amino Acid-Polyamine-Organocation (APC) Family	0.58	0.8	1	-0.34	0.89	1	1	0.066	1	1	1	0.52	0.94	0.54	0.96	0.00055	9.1e-05	0	2.6e-05	0	6.9e-05	0.00019	0	0.00051	2.9	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
9.B.143.2.2	The 6 TMS DUF1275/Pf06912 (DUF1275) Family	0.99	0.99	1	-0.0059	0.72	1	1	0.19	1	1	1	0.62	0.95	0.66	0.97	0.00013	3.6e-05	0	3.6e-05	0	6.8e-05	3.4e-05	0	5.9e-05	-0.082	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
1.B.42.1.2	The Outer Membrane Lipopolysaccharide Export Porin (LPS-EP) Family	0.49	0.8	1	-0.44	0.89	1	1	-0.071	1	1	1	0.54	0.93	0.64	0.96	0.0015	0.00042	0	7.6e-05	0	0.00015	0.00095	0	0.0025	3.6	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
2.A.73.1.1	The Short Chain Fatty Acid Uptake (AtoE) Family	0.55	0.8	1	-0.37	0.94	1	1	0.033	1	1	1	0.48	0.93	0.54	0.95	0.00024	3.9e-05	0	9.2e-06	0	2.2e-05	8.7e-05	0	0.00023	3.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
4.D.3.1.6	The Glycan Glucosyl Transferase (OpgH) Family	0.6	0.8	1	-0.33	0.69	1	1	0.2	1	1	1	0.53	0.93	0.57	0.96	0.00065	0.00014	0	3.1e-05	0	5.5e-05	0.00032	0	0.00084	3.4	4 / 18 (22%)	3 / 11 (27%)	1 / 7 (14%)	0.273	0.143
2.A.86.1.1	The Autoinducer-2 Exporter (AI-2E) Family (Formerly the PerM Family. TC #9.B.22)	0.32	0.8	1	-0.65	0.16	1	1	-0.89	0.53	1	1	0.46	0.92	0.51	0.94	0.00024	4.1e-05	0	2.7e-06	0	8.9e-06	1e-04	0	0.00025	5.2	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
3.A.1.3.4	The ATP-binding Cassette (ABC) Superfamily	0.41	0.8	1	-0.53	0.53	1	1	-0.34	1	1	1	0.53	0.94	0.53	0.96	0.00059	0.00013	0	2.6e-05	0	6.1e-05	3e-04	0	0.00074	3.5	4 / 18 (22%)	2 / 11 (18%)	2 / 7 (29%)	0.182	0.286
3.A.1.3.10	The ATP-binding Cassette (ABC) Superfamily	0.56	0.8	1	-0.36	0.91	1	1	0.052	1	1	1	0.49	0.93	0.54	0.96	4e-04	6.6e-05	0	1.6e-05	0	3.7e-05	0.00015	0	0.00038	3.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.38.1.1	The K⁺ Transporter (Trk) Family	0.55	0.8	1	-0.37	0.92	1	1	0.049	1	1	1	0.5	0.94	0.56	0.96	0.00049	0.00014	0	3.6e-05	0	6.9e-05	0.00029	0	0.00077	3	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
5.A.3.1.2	The Prokaryotic Molybdopterin-containing Oxidoreductase (PMO) Family	0.55	0.8	1	-0.37	0.91	1	1	0.057	1	1	1	0.53	0.93	0.58	0.95	0.00051	0.00014	0	3.7e-05	0	6.3e-05	3e-04	0	0.00079	3	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
1.B.1.1.2	The General Bacterial Porin (GBP) Family	0.28	0.8	1	-0.71	0.23	1	1	-0.75	0.53	1	1	0.49	0.92	0.53	0.96	0.00028	4.7e-05	0	5.4e-06	0	1.8e-05	0.00011	0	0.00025	4.3	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286
2.A.28.2.6	The Bile Acid:Na⁺ Symporter (BASS) Family	0.5	0.8	1	0.32	0.48	1	1	0.34	1	1	1	0.55	0.94	0.46	0.95	7e-04	0.00051	0.00046	6e-04	0.00053	0.00054	0.00037	0.00034	0.00039	-0.7	13 / 18 (72%)	8 / 11 (73%)	5 / 7 (71%)	0.727	0.714
2.A.72.1.1	The K⁺ Uptake Permease (KUP) Family	0.4	0.8	1	-0.52	0.62	1	1	-0.27	1	1	1	0.49	0.93	0.52	0.95	0.00026	7.3e-05	0	1.9e-05	0	3.8e-05	0.00016	0	0.00033	3.1	5 / 18 (28%)	3 / 11 (27%)	2 / 7 (29%)	0.273	0.286
1.A.104.1.1	The Proposed Flagellar Biosynthesis Na+ Channel. FlaH (FlaH) Family	0.53	0.8	1	-0.39	0.98	1	1	-0.014	1	1	1	0.53	0.94	0.56	0.96	0.00028	4.7e-05	0	9.5e-06	0	2.3e-05	0.00011	0	0.00028	3.5	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
2.A.35.1.8	The NhaC Na⁺:H⁺ Antiporter (NhaC) Family	0.49	0.8	1	0.29	0.49	1	1	0.36	1	1	1	0.59	0.95	0.65	0.97	0.00036	0.00014	0	2e-04	0	5e-04	4.9e-05	0	9.2e-05	-2	7 / 18 (39%)	4 / 11 (36%)	3 / 7 (43%)	0.364	0.429
3.A.1.5.3	The ATP-binding Cassette (ABC) Superfamily	0.58	0.8	1	-0.34	0.86	1	1	0.087	1	1	1	0.52	0.94	0.56	0.96	0.00089	0.00015	0	3.9e-05	0	8.8e-05	0.00032	0	0.00084	3	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.A.5.1.1	The General Secretory Pathway (Sec) Family	0.49	0.8	1	-0.44	0.92	1	1	-0.053	1	1	1	0.53	0.94	0.57	0.96	0.0016	0.00027	0	3.4e-05	0	7.8e-05	0.00064	0	0.0017	4.2	3 / 18 (17%)	2 / 11 (18%)	1 / 7 (14%)	0.182	0.143
3.D.6.1.4	The Putative Ion (H⁺ or Na⁺)-translocating NADH:Ferredoxin Oxidoreductase (NFO or RNF) Family	0.33	0.8	1	-0.65	0.18	1	1	-0.89	0.53	1	1	0.45	0.92	0.46	0.94	0.00056	9.3e-05	0	2.7e-06	0	8.9e-06	0.00024	0	0.00061	6.5	3 / 18 (17%)	1 / 11 (9.1%)	2 / 7 (29%)	0.0909	0.286

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Methods:

P Fisher's exact test: differences in detection rate were detected by Fisher's exact test.

P Welch's t-test (sqrt): Differentially abundant functions were identified by Welch's t-test.

P Welch's t-test (clr): Differentially abundant functions were identified by Welch's t-test.

Glossary:

clr: Centered log-ratio transformation. P Welch's ANOVA (sqrt): Welch's ANOVA p-values (Anova was run on sqrt transformed abundances). It is an alternative to the classic ANOVA and can be used even if the samples have unequal variances and/or unequal sample sizes. P Welch's t-test (sqrt): Welch's T-test p-values (run on sqrt transformed abundances). It is an alternative to the classic Student's t-test and is more reliable if the data violates the assumption of homogeneity of variances and/or if the study conditions have unequal sample sizes. P Welch's ANOVA (clr): Welch's ANOVA p-values (Anova was run on clr transformed abundances). It is an alternative to the classic ANOVA and can be used even if the samples have unequal variances and/or unequal sample sizes. P Welch's t-test (clr): Welch's T-test p-values (run on clr transformed abundances). It is an alternative to the classic Student's t-test and is more reliable if the data violates the assumption of homogeneity of variances and/or if the study conditions have unequal sample sizes. P lmer XXX (clr): P-value of Linear Mixed-Effects Regression on clr transformed abundances, testing significance of XXX effect. Varying intercepts per subjects are used to control for repeated measures. Pbonf: Bonferroni corrected p-value. FDR: False Discovery Rate q-value. Mean Pos: Mean abundance in positive samples. Positive samples: The number and percentage of samples in which each function has been detected. Positive XXX: The number and percentage of positive samples in study group XXX. Positive_XXX_percent: Percentage of positive samples in study group XXX. P Welch's t-test (Aldex2): Expected P value of Welch’s t-test computed by Aldex2. FDR Welch's t-test (Aldex2): Expected Benjamini-Hochberg corrected P value for Welch’s t-test. P Wilcoxon rank test (Aldex2): Expected P value of Wilcoxon rank test computed by Aldex2. FDR Wilcoxon rank test (Aldex2): Expected Benjamini-Hochberg corrected P value of Wilcoxon test. P Kruskal-Wallace test (Aldex2): Expected P value of Kruskal-Wallace test. FDR Kruskal-Wallace test (Aldex2): Expected Benjamini-Hochberg corrected P value of Kruskal-Wallace test. P GLM test (Aldex2): Expected P value of generalized linear model. FDR GLM test (Aldex2): Expected Benjamini-Hochberg corrected P value of generalized linear model.

Differentially Abundant Microbial Functions

The following plots present the distribution of the top most differentially abundant microbial functions across all applied statistical analysis. Plots are ordered alphabetically.

Glossary:

"Rel. Abundance": Relative abundance data; "Rel. Abundance (sqrt)": sqrt (Hellinger) transformed relative abundances; "Rel. Abundance (clr)": centered log-ratio (clr) transformed read counts.